Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Multi-enzyme system
Reexamination Certificate
2008-09-23
2008-09-23
Redding, David A (Department: 3723)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Multi-enzyme system
C435S176000, C435S179000, C435S181000, C435S283100, C435S289100
Reexamination Certificate
active
10287440
ABSTRACT:
Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
REFERENCES:
patent: 3650900 (1972-03-01), Levin et al.
patent: 4331767 (1982-05-01), Nakajima et al.
patent: 5051184 (1991-09-01), Taylor
patent: 5181999 (1993-01-01), Wiktorowicz
patent: 5200334 (1993-04-01), Dunn et al.
patent: 5474915 (1995-12-01), Dordick et al.
patent: 5618933 (1997-04-01), Dordick et al.
patent: 5622819 (1997-04-01), Herman
patent: 5637469 (1997-06-01), Wilding et al.
patent: 5716825 (1998-02-01), Hancock et al.
patent: 5824526 (1998-10-01), Avnir et al.
patent: 5854030 (1998-12-01), Dordick et al.
patent: 6042709 (2000-03-01), Parce et al.
patent: 6063589 (2000-05-01), Kellogg et al.
patent: 6261813 (2001-07-01), Khmelnitsky et al.
patent: 6303290 (2001-10-01), Liu et al.
patent: 6475805 (2002-11-01), Charm et al.
patent: 6582969 (2003-06-01), Wagner et al.
patent: 7172682 (2007-02-01), Dordick et al.
patent: 2001/0055797 (2001-12-01), Conroy et al.
patent: 2002/0020931 (2002-02-01), Stowell et al.
patent: 2002/0028506 (2002-03-01), Ho et al.
patent: 2002/0185184 (2002-12-01), O'Connor et al.
patent: WO 00/62051 (2000-10-01), None
Arenkov, P., et al., “Protein microchips: Use for immunoassay and enzymatic reactions,”Anal. Biochem., 278: 123-131 (2000).
Cane, D.E., et al. “The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases,”Chemisty&Biology, 6(12): R319-R325 (1999).
Drott, J., et al. “Porous Silicon as the carrier matrix in microstructured enzyme reactors yielding high enzyme activities,”J. Micromech. Microeng., 7: 14-23 (1997).
Gill, I., et al., “Encapsulation of biologicals within silicate, siloxane, and hybrid sol-gel polymers: an efficient and generic approach,”J. Am. Chem. Soc., 120(34): 8587-8598 (1990).
Gill, I., et al., “Bioencapsulation within synthetic polymers (Part 1): sol-gel encapsulated biologicals,”TIBTECH, 18: 282-296 (2000).
Hadd, A.G., et al., “Microchip device for performing enzyme assays,”Anal. Chem., 69:3407-3412 (1997).
Haswell, S.J., et al., “Chemical and biochemical microreactors,”Trends Anal., 19: 389-395 (2000).
Katz, L., “Manipulation of modular polyketide synthases,”Chem. Rev., 97: 2557-2575 (1997).
Khosla, C., “Harnessing the biosynthetic potential of modular polyketide synthases,”Chem. Rev., 97: 2577-2590 (1997).
Kim, Y., et al., “Stable sol-gel microstructured and microfluidic networks for protein patterning,”Biotechnology&Bioengineering, 73(5): 331-337 (2001).
Kim, J., et al., “Protease-containing silicates as active antifouling materials,”Biotechnology Prog., 18(3): 551-555 (2002).
Laurell, T., et al. “Silicon wafer integrated enzyme reactors,”Biosensors&Bioelectronics, 10(3/4):289-299 (1995).
Leadlay, P.F., “Combinatorial approaches to polyketide biosynthesis,”Curr. Op. Chem. Biol., 1:162-168 (1997).
Nagy, G., et al., “Amperometric microcell for enzyme activity measurements,”Anal Chem., 70: 2156-2162 (1998).
Novick, S.J., et al., “Investigating the effects of polymer chemistry on activity of biocatalytic plastic materials,”Biotechnology and Bioengineering, 68(6): 665-671 (2000).
Novic, S.J., et al., “Preparation of active and stable biocatalytic hydrogels for use in selective transformations,”Chem. Mat., 10:955-958 (1998).
Park, C.B., et al., “Sol-gel encapsulated enzyme arrays for high-throughput screening of biocatalytic activity,”Biotechnology&Bioengineering, 78(2): 229-235 (2002).
Sergeeva, M.V., et al., “Peptide synthesis using proteases dissolved in organic solvents,”Enzyme Microbial Technogy, 20: 623-628 (1997).
Wang, P., et al., “Biocatalytic plastics as active and stable materials for biotransformations,”Nature: Biotechnology, 15: 789-793 (1997).
Clark Douglas S.
Dordick Jonathan S.
Kim Jung-bae
Sherman David H.
Srinivasan Aravind
Darpa
Hamilton Brook Smith & Reynolds P.C.
Redding David A
Rensselaer Polytechnic Institute
The Regents of the University of California
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