In vitro maturation and fertilization of mammalian oocytes

Details In vitro maturation and fertilization of mammalian oocytes In vitro maturation and fertilization of mammalian oocytes

1997-03-27

1999-03-16

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

435 11, 435 13, 435366, 435377, C12N 500, A01N 102

Patent

active

058829288

ABSTRACT:
An in vitro fertilization method comprising culturing mammalian immature oocytes obtained from a mammalian ovary very early in the mammal's menstrual cycle in an oocyte maturation inhibitor-containing culture, removing the inhibitor and maturing and fertilizing the oocytes to produce embryos for subsequent uterine-implantation. The method allows for more convenient, efficacious, safer, less expensive and timely implantation of a plurality of embryos in the uterus to enhance pregnancy success rate. Cryopreservation of the immature oocytes offers enhanced benefits Preferably, the mammal is human and the inhibitor is dbcAMP.

REFERENCES:
Tornell et al., "Effect of cyclic AMP on the isolated human oocyte-cumulus complex", Human Reproduction 8 (5) : 737-739 (1993).
Son et al., "Effects of 1,2-propanediol and freezing-thawing on the in vitro developmental capacity of human immature oocytes", Fertility and Sterility 66 (6) : 995-999 (1996).
Petr et al., "Effect of Testosterone and dibutyryl c-AMP on the meiotic competence in pig oocytes of various size catagories" Theriogenology 46 : 97-108 (1996).
Cha et al., "Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor ooocyte program", Fertility and Sterility 55 (1) : 109-13 (1991).
Schroeder, A.C. & Eppig, J.J., Dev. Biol. 102: 493-497 (1984).
Cho, W.K., Stern, S. & Biggers, J.D., J. Exp. Zool. 187: 383-386 (1974).

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