In vitro management of ammonia's effect on glycosylation of cell

Details In vitro management of ammonia's effect on glycosylation of cell In vitro management of ammonia's effect on glycosylation of cell

1990-06-05

1992-03-17

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

43524027, 43524031, C12P 2108, C12N 512, C12N 502

Patent

active

050968165

ABSTRACT:
This invention relates generally to the field of cell culture. The invention presents pH based methods for controlling the glycosylation patterns of cell products, particularly proteins produced by cells in vitro, preferably via a multi-level pH control strategy. Preferably, this invention is applied to manage the effect of high level of ammonia on the glycosylation of cell products. The glycosylation affected is preferably that of terminal sialylations of the oligosaccharide of glycoproteins.

REFERENCES:
patent: 4879222 (1989-11-01), Alderman et al.
Thorens, et al. Chloroquime and Ammonium Chloride Prevent Terminal Glycosylation of Immunoglobulins in Plasma Cells . . . Nature vol. 321, pp. 618-620, Jun. 5, 1986.
Dean et al. Effects of Exogenous Amines on Mammalian Cells, with Particular Reference to Membrane Flow Biochemical Journal vol. 217, pp. 27-40, 1984.

Affiliated with

Also associated with

Rating

In vitro management of ammonia's effect on glycosylation of cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In vitro management of ammonia's effect on glycosylation of cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro management of ammonia's effect on glycosylation of cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1475442