In vitro generation of differentiated neurons from cultures of m

Details In vitro generation of differentiated neurons from cultures of m In vitro generation of differentiated neurons from cultures of m

1997-05-07

2000-03-21

Allen, Marianne P.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

435325, 435368, 435353, C12N 506

Patent

active

06040180&

ABSTRACT:
The present invention reveals in vitro cultures of region-specific, terminally differentiated, mature neurons derived from cultures of mammalian multipotential CNS stem cells and an in vitro procedure by which the differentiated neurons may be generated. The procedure involves the culturing of multipotential CNS stem cells from a specific region in a chemically defined serum-free culture medium containing a growth factor; replacing the medium with growth factor-free medium; harvesting the stem cells by trypsinization; plating the stem cells at a density of between 100,000 to 250,000 cells per square centimeter; and culturing the stem cells in a glutamic acid-free chemically defined serum-free culture medium.

REFERENCES:
patent: 5580777 (1996-12-01), Bernard et al.
patent: 5851832 (1998-12-01), Weiss et al.
Davis, A.A. & Temple, S., Nature 372, 263-266 (1994).
Reynolds, B., Tetzlaff, W. & Weiss, S., J. Neurosci. 12, 4565-4574 (1992).
Ray, J., Peterson, D., Schinstine, M. & Gage, F., Proc. Natl. Acad. Sci. USA 90, 3602-3606 (1993).
Vicario-Abejon, C., Johe, K., Hazel, T., Collazo, D. & McKay, R., Neuron 15, 105-114 (1995).
Sabate, O., Horellou, P., Vigne, E., Colin, P., Perricaudet, M., Buc-Caron, M.-H. & Mallet, J., Nature Genetics 9, 256-260 (1995).
Svendsen, C.N. & Rosser, A.E., Trends in Neuroscience 18, 465-466 (1995).
Gage, F.H., Ray, J. & Fisher, L.J., Annu. Rev. Neurosci. 18, 159-192 (1995).
Gage, F.H., Coates, P.W., Palmer, T.D., Kuhn, H.G., Fisher, L.J., Suhonen, J.O., Peterson, D.A., Suhr, S.T. & Ray, J., Proc. Natl. Acad. Sci. USA 92, 11879-11883 (1995).
Svendsen, C.N., Fawcett, J.W., Bentlage, C. & Dunnett, S.B., Exp. Brain Res. 102, 407-414 (1995).
Hermanson, M., Olsson, T., Westermark, B., & Funa K., Exp. Brain Res. 102, 415-422 (1995).
Kilpatrick, T.J., Richards, L.J., and Bartlett, P.F., Mol. Cell. Neurosci. 6, 2-15 (1995).
Ray, J., and Gage, F.H., J. Neurosci. 14, 3548-3564 (1994).
Gritti, A., et al., J. Neurosci. 16, 1091-1100 (1996).
Vescovi, A.L., Reynolds, B.A., Fraser, D.D., and Weiss, S., Neuron 11, 951-966 (1993).
Ahmed, S., Reynolds, B.A., and Weiss, S., J. Neurosci. 15, 5765-5778 (1995).
Kilpatrick, T.J. and Bartlett, P.F., J. Neurosci. 15, 3653-3661 (1995).
Baetge, E.E., Ann. N.Y. Acad. Sci. 695, 285 (1993).
Bartlett, P.F. et al., Clin. Exp. Pharm. Physiol. 22, 559-562 (1995).
Palmer, T.D., Takahashi, J., and Gage, F.H., Mol. Cell. Neurosci. 8, 389-404 (1997).
Finley, M.F.A., Kulkarni, N., and Huettner, J.E., J. Neurosci. 16, 1056-1065 (1996).
Schlaggar, B.L. and O'Leary, D.D.M., Science 252, 1556-1560 (1991).
Rakic, P., Proc. Natl. Acad. Sci. USA 92, 11323-11327 (1995).
McConnell, S.K., Neuron 15, 761-768 (1995).
Temple and Qian, Curr. Opin. Neurobiol. 6, 11-17 (1996).
Johe, K.K., Hazel, T.G., Muller, T., Dugich-Djordevic, M.M., and McKay, R.D.G., Genes Dev. 10, 3129-3140 (1996).
Lumsden, A. And Krumlauf, R., Science 274, 1109-1115 (1996).
McKay, R. Science 276, 66-71 (1997).
Morrison, S.J., Shah, N.M., and Anderson, D.J., Cell 88, 287-298 (1997).
Stemple, D.L. and Mahanthappa, N.K., Neuron 18, 1-4 (1997).
Weiss, S., Reynolds, B.A., Vescovi, A.L., Morshead, C., Craig, C.G., van der Kooy, D., Trends Neurosci. 19, 387-393 (1996).
Brustle, O. and McKay, R.D.G., Curr. Opin. Neurobiol. 6, 688-695 (1996).
Schinstine, M. and Iacovitti, L., Exp. Neurol. 141, 67-78 (1996).
Svendsen, C.N., Clarke, D.J., Rosser, A.E., and Dunnett, S.B., Exp. Neurol. 137, 376-388 (1996).
Weiss, S., Dunne, C., Hewson, J., Wohl, C., Wheatley, M., Peterson, A.C., and Reynolds, B.A., J. Neurosci. 16, 7599-7609 (1966).
Reynolds, B.A. and Weiss, S., Dev. Biol. 175, 1-13 (1996).
Feldman, D.H., Thinschmidt, J.S., Peel, A.L., Papke, R.L., and Reier, P.J., Exp. Neurology 140, 206-217 (1996).
Strubing, C., Ahnert-Hilger, G., Shan, J., Wiedenmann, B., Hescheler, J., and Wobus, A.M., Mech. Dev. 53, 275-287 (1995).
Qian, X., Davis, A.A., Goderie, S.K., and Temple, S., Neuron 18, 81-93 (1997).
Von Visger, Experimental Neurology 128: 34-40, 1994.
Vicario-Abejon, Neuron 15: 105-114, 1995.
Johe, Genes and Development 10: 3129-3140, 1996.

Affiliated with

Also associated with

Rating

In vitro generation of differentiated neurons from cultures of m does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In vitro generation of differentiated neurons from cultures of m, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro generation of differentiated neurons from cultures of m will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-729774