In vitro fertilisation

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S002000, C435S455000

Reexamination Certificate

active

07547541

ABSTRACT:
The present invention relates to a method and a system for producing a mammalian pre-embryo and a stem cell having a better quality than prior art methods. The system comprises means for obtaining a mammalian oocyte, and means for obtaining a mammalian spermatozoa, and an apparatus having at least two separate air-tight chambers, for which the oxygen tension of one chamber may be changed independent of the oxygen tension of the other chamber, said at least two separate air-tight chambers constitute a main chamber and at least one residence chamber. The method for in vitro producing a mammalian pre-embryo comprising the steps: a1) providing a mammalian oocyte, a2) providing a mammalian spermatozoa, b) culturing the oocyte and the spermatozoa, c) fertilizing the oocyte with the spermatozoa obtaining a fertilized oocyte, and d) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo wherein at least one of the steps a1) or a2) is conducted at an oxygen tension below 15%, or e) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo, wherein the culture is performed at an oxygen tension allowing cultivation of the cells and wherein at least one of the steps comprises a change in the oxygen tension. Stem cells are produced from the multicellular pre-embryo.

REFERENCES:
patent: 4262091 (1981-04-01), Cox
patent: 4892830 (1990-01-01), Findley et al.
patent: 5169217 (1992-12-01), Orchard et al.
patent: 5219215 (1993-06-01), Akagawa et al.
patent: 5730777 (1998-03-01), Petersen et al.
patent: 5837543 (1998-11-01), Conway-Myers et al.
patent: 5882928 (1999-03-01), Moses
patent: 6050935 (2000-04-01), Ranoux et al.
patent: 6110741 (2000-08-01), Hearn et al.
patent: 6140121 (2000-10-01), Ellington et al.
patent: 6184035 (2001-02-01), Csete et al.
patent: 6399375 (2002-06-01), Vajta
patent: 2001/0028878 (2001-10-01), Lindenberg
patent: 2001/0049829 (2001-12-01), DeSousa et al.
patent: 2002/0068358 (2002-06-01), Campbell et al.
patent: 1 018 547 (2000-07-01), None
patent: WO 98/30676 (1998-07-01), None
patent: WO 99/67364 (1999-12-01), None
patent: WO 99/67365 (1999-12-01), None
patent: WO 00/32140 (2000-06-01), None
patent: WO 00/38583 (2000-07-01), None
patent: WO 01/64845 (2001-09-01), None
patent: WO 01/94552 (2001-12-01), None
Burkman L. J. A Microperfusion Chamber for study of Mammalian Spermatozoa. 1998. Journal of Andrology, v. 9, p. 102-108.
James W. Catt et. al., “Toxic effects of oxygen on human embryo development”,Human Reproduction, vol. 15, (Suppl. 2), pp. 199-206, Jul. 2000.
James A. Thomson et. al., “Human embryonic stem cell and embryonic germ cell lines”,Trends in Biotechnology, vol. 18, No. 2, pp. 53-57, Feb. 2000.
J. F. Griveau et. al., “Influence of oxygen tension on reactive oxygen species production and human sperm function”,International Journal of Andrology, vol. 20, No. 4, pp. 195-200, Aug. 1997.
John J. Eppig et. al., “Factors Affecting the Developmental Competence of Mouse Oocytes Grown In Vitro: Oxygen Concentration”,Molecular Reproduction and Development, vol. 42, No. 4, pp. 447-456, Dec. 1995.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

In vitro fertilisation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In vitro fertilisation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro fertilisation will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4063898

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.