Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1987-12-30
1993-05-25
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352403, 43524025, C12N 500
Patent
active
052139794
ABSTRACT:
The disclosure herein describes a bovine embryo culture medium which includes epithelial cells from the oviduct of a donor animal. Additionally, a process for maturing immature bovine oocytes in an in vitro bovine culture medium containing epithelial cells from a donor oviduct is described. As an alternative to co-culture of the embryos with the epithelial cells, it is described how a culture medium can be conditioned for the culture of bovine embryos by the culture of epithelial cells therein which are later removed.
REFERENCES:
Eyestone et al., Thermogenology 27(1):228 (1987).
Freshney, "Culture of Animal Cells", Alan R. Liss, Inc., New York, (1983), pp. 78, 7, 106-110, 113-116.
Thibault, "La Culture In Vitro de L'Oeuf de Fache", Annls. Biochim. Biophys., vol. 6, pp. 159-164 (1966).
Camous, et al., "Cleavage Beyond the Block Stage and Survival After Transfer of Early Bovine Embryos Cultures with Trophoblastic Vesicles", J. Reprod. Fert., vol. 72, pp. 479-485 (1984).
Eyestone, et al., "Culture of One-and Two- Cell Bovine Embryos to the Blastocyst Stage in the Ovine Oviduct", Theriogenology, vol. 28, pp. 1-7 (1987).
Wright, et al., "Aspects of In Vitro Fertilization and Embryo Culture in Domestic Animals", J. Animal Science, vol. 53, No. 3, pp. 702-729 (1981).
Tervit, (H. R.), et al., "Successful Culture In Vitro of Sheep and Cattle Ova", J. Reprod. Fert., vol. 30, pp. 493-497 (1972).
Wright, et al., "Successful Culture In Vitro of Bovine Embryos to the Blastocyst Stage", Biol. Repr., vol. 14, pp. 157-162 (1976).
Rexroad, et al., "Co-culture of Sheep Ova and Cells from Sheep Oviduct", Theriogenology, vol. 25, p. 187 (1986).
Gandolfi, et al., "Role of Somatic Cells in the Long Term Culture of Ovine Embryos," Society for the Study of Fertility-Annual Conference, Abstract 111 (1986).
Eyestone Willard
First Neal L.
Appleby Vanessa L.
Chambers Jasemine C.
Krafte Jill H.
W. R. Grace & Co.,-Conn.
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