Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1984-09-14
1988-07-12
Warren, Charles F.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524024, C12N 500
Patent
active
047570172
ABSTRACT:
In vitro cell culture systems for anchorage-dependent mammalian cells using solid substrates of mitogenic calcium compounds, such as hydroxyapatite and tricalcium phosphate forms of calcium phosphate and calcium carbonate. The calcium solid substrates can be in the form of granules with a particle size of at least 0.050 mm. or in the form of solid bodies and may be either porous or non-porous. Unique features of cells cultured in the stated in vitro cell culture systems include the growth of cells in layers many cells thick, growth of cells that maintain their phenotype and the ability to culture cells for extended periods of time.
REFERENCES:
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Akao et al, "Dense Polycrystalline Beta-Tricalcium Phosphate for Prosthetic Applications" Journal of Materials Science 17 pp. 343-346 (1982).
Cheung et al, "Phagocytosis of Hydroxyappatite or Calcium Pyrophosphate Dihydrate Crystals by Rabbit Articular Chondrocytes . . . ", Proceedings of the Society for Experimental Biology and Medicine 173, p. 181-189 (1983).
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Knox Gail F.
MCW Research Foundation, Inc.
Warren Charles F.
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