In vitro assay for quantitating secreted antibodies in...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C530S380000, C530S386000, C530S387100, C530S412000, C530S413000, C530S866000, C530S861000, C530S862000, C530S863000, C435S002000, C435S340000, C435S326000, C435S327000, C435S377000, C435S383000

Reexamination Certificate

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10412011

ABSTRACT:
The present invention provides methods of quantitating recent secreted antigen specific antibodies from supernatant of antibody secreting cells (ASC) in vitro culture for evaluation of vaccine or antigen induced antigen specific antibody secretion without ex vivo antigen stimulation.

REFERENCES:
patent: 5653986 (1997-08-01), Morris et al.
Lane et al., Journal of Experimental Medicine. vol. 154, Oct. 1981, pp. 1043-1057.
Peters et al., The Journal of Immunology. vol. 130, No. 2, Feb. 1983, pp. 678-680.
Rebelatto et al., Veterinary Immunology and Immunopathology. vol. 83. 2001. pp. 93-105.
Volkman et al., Proc. Natl. Acad. Sci. vol. 78, No. 4, pp. 2528-2531, Apr. 1981.

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