Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-03-17
1998-11-17
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 29, 435 34, 436 63, 436163, 436172, C12Q 168, C12Q 104, G01N 3348
Patent
active
058374710
ABSTRACT:
Disclosed is a method to evaluate the carcinogenicity of a compound using a transformation assay. The method includes contacting a compound to be tested for carcinogenicity with a test cell. The test cell has a defect in a protective cellular mechanism selected from the group of a defect in a DNA damage repair mechanism, a defect in cell cycle control, and a defect in the ability to prevent damage induced by oxygen free radicals. Cell growth is scored to identify the presence or absence of a transformation characteristic. The development of such a transformation characteristic indicates that the compound being tested is carcinogenic. Further embodiments include a method to identify tissue-specific carcinogens, a method to identify the biochemical mechanism of carcinogenicity of a compound, and a method to evaluate the anticarcinogenicity of a compound.
REFERENCES:
patent: 4302535 (1981-11-01), Skopek et al.
patent: 4753874 (1988-06-01), Calos
patent: 4808532 (1989-02-01), Stampfer
patent: 4885238 (1989-12-01), Reddel et al.
patent: 4904595 (1990-02-01), Gierthy
patent: 5180666 (1993-01-01), States et al.
patent: 5273880 (1993-12-01), Schiesti
patent: 5356806 (1994-10-01), Harris et al.
patent: 5429948 (1995-07-01), Crespi et al.
patent: 5506131 (1996-04-01), Harris et al.
Boyer et al., Cancer Res., 50:2593-2598, May, 1990.
Chernova et al., "The Role of p53 in Regulating Genomic Stability when DNA and RNA Synthesis are Inhibited," TIBS, 20, Oct. 1995.
Doetsch, TIBS, 20:384-386, Oct. 1995.
Greenblatt et al., Cancer Res., 54:4855-4878, Sep. 1994.
Jackson et al., TIBS, 20:412-415, Oct. 1995q.
Kastan et al., Cancer Res., 51:6304-6311, Dec. 1991.
Kastan et al., Cell, 71:587-597, Nov. 1992.
Kat et al., Proc. Natl. Acad. Sci. USA, 90:6424-6428, Jul. 1993.
Lavin et al., "Relationship of the Ataxia-Telangiectasia Protein ATM to Phosphoinositide 3-Kinase", TIBS, 20, Oct. 1995.
Leadon et al., Proc. Natl. Acad. Sci. USA, 90:10499-10503, Nov. 1993.
Lindahl et al., TIBS, 20:405-411, Oct. 1995.
Papadopoulos et al., Science, 263:1625-1629, Mar. 1994.
Sancar, Annu. Rev. Genetics, 29:69-105, 1995.
Stevnsner et al., 1995, Carcenogenesis, 16(11):2855-2858.
Shiloh et al., "In Vitro Phenotype of Ataxia-Telangiectasia (AT) Fibroblast Strains: Cluese to the Nature of the `AT DNA Lesion` and the Molecular Defect in AT", in Ataxia-Telangiectasia: Genetics, Neuropathology, and Immunology of a Degenerative Disease of Childhood, pp. 111-121, Alan R. Liss, Inc., 1985.
Stefanini et al., Am. J. Hum. Genet., 53:817-821, 1993.
Steinman et al., Oncogene, 9:3389-3396, 1994.
Walker, TIBS, 20:416-420, Oct. 1995.
Degen Nancy
Viratest Carcinogen Monitoring Ltd.
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