In vitro amplification/expansion of CD34.sup.+ stem and progenit

Details In vitro amplification/expansion of CD34.sup.+ stem and progenit In vitro amplification/expansion of CD34.sup.+ stem and progenit

1993-10-28

1997-02-04

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of co-culturing cells

435385, 435386, 424 937, C12N 500, A01N 6300, A01N 6500

Patent

active

055997031

ABSTRACT:
The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34.sup.+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34.sup.+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34.sup.+ stem and progenitor cells which can be used in human therapeutics.

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