Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-05-02
2006-05-02
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069100, C435S091200, C536S024330
Reexamination Certificate
active
07037682
ABSTRACT:
The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5′ oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio-labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti-HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.
REFERENCES:
patent: 5492817 (1996-02-01), Thompson et al.
patent: WO89/06700 (1989-07-01), None
patent: WO 94/05809 (1994-03-01), None
patent: WO 94/05812 (1994-03-01), None
patent: WO 95/17523 (1995-06-01), None
patent: WO 96/10649 (1996-04-01), None
Chayama, et. al., Emergence and Takeover of YMDD Motif Mutant Hepatitis B Virus During Long-Term Lamivudine Therapy and Re-Takover by Wild Type After Cessation of Therapy, Jun. 1998, Hepatology, vol. 27, No. 6, 1711-1716.
Wang and Seeger, The Reverse Transcriptase of Hepatitis B Virus Acts as a Protein Primer for Viral DNA Synthesis, Cell, Nov. 1992, vol. 71, 663-670.
Li et al. Expression of an enzymatically active polymerase of human hepatitis B virus in a coupled transcription-translation system (1999) Biochemistry and Cell Biology, 77:2 pp. 119-126.
Martemyanov, K.A., et al., “Direct Expression of PCR Products in a Cell-Free Transcription/Translation System: Synthesis of Antibacterial Peptide Cecropin,”FEBS Letters 414(1997) pp. 268-270.
Sprengel, Isolation and Characterization of a Hepatitis B Virus Endemic in Herons, Journal of Virology, vol. 62, No. 10, p. 3832-3839, Oct. 1988.
Howe, A., et al.,Biochemical and Biophysical Research Communications, “Duck hepatitis B virus polymerase produced by in vitro transcription and translation possesses DNA and reverse transcriptase activities”, vol. 189, No. 2, pp. 1170-1176, Dec. 15, 1992.
Chen Wei Ning
Leong Ai Lin
Lim Gek Keow
Oon Chong Jin
Chen Shirley
Government of the Republic of Singapore
Housel James
Salvoza M. Franco
Wilson Sonsini Goodrich & Rosati
LandOfFree
In vitro activity assay for human hepatitis B virus (HBV)... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with In vitro activity assay for human hepatitis B virus (HBV)..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro activity assay for human hepatitis B virus (HBV)... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3647630