In situ transcription in cells and tissues

Details In situ transcription in cells and tissues In situ transcription in cells and tissues

1991-03-18

1992-12-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 91, 4351722, 4351723, 4353201, 935 16, 935 18, 935 19, 935 39, 935 93, 536 27, 436 27, C12Q 168, C12P 1934, C12N 1500, G01N 3324

Patent

active

051680386

ABSTRACT:
In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with transverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.

REFERENCES:
patent: 4886741 (1989-12-01), Schwartz et al.
Maniaitis, T. et al., "Molecular Cloning" A Laboratory Manual. pp. 213-216 and pp. 224-235.
Pardue et al. M. L. in: Hames B & Higgins S. "Nucleic Acid Hybridization" A practical Approach. Chap. 8, pp 179-202. (1987).
Eberwine et al. "Isolation at Enzyme c DNA Clones by enzyme Immunodetection. in PNAS" 84:1449-1453 (1987).
Sciki et al. "Primer directed Enzymatic Amplification of DNA with a thermostable DNA Polymerase Science" 239:487 (1988).
Baltimore. "Vial RNA-dependent DNA polymerase" Nature 226:1209. (1970).
Tecott L. "In situ transcription: specific. synthesis of complementary DNA in fixed tissue section" Science 240:1661-1664 (1988).
Shibata et al. "Analysis of DNA sequence on Forty-Year-Old Paraffin-embedded Thin-Tissue Sections". Cancer Res. 48:4564 (1988).

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