Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1993-02-16
1994-11-15
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435290, 435316, 435809, 359398, 422 99, 422102, 422104, C12M 140
Patent
active
053647904
ABSTRACT:
A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover over the sample, and a retaining assembly fastened to the slide and to the cover to retain and seal a reaction mixture against the sample during thermal cycling. The retaining assembly includes a rigid ring on a rim portion of the cover, a cross beam having spaced parallel rails joined by opposite flat ends, and a pair of clips which are pressed over the ends and opposite sides of the slide to fasten the cross beam and cover to the slide.
REFERENCES:
patent: 3503665 (1970-03-01), Carter
patent: 3879106 (1975-04-01), McCormick
patent: 3883398 (1975-05-01), Ono
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4985206 (1991-01-01), Bowman et al.
patent: 5038852 (1991-08-01), Johnson et al.
Katherine A. Staskus et al, In Situ Amplification of Visna Virus DNA in Tissue Sections Reveals a Reservoir of Latently Infected Cells, Microbial Pathogenesis, 11, pp. 67-76 (1991).
Gerard J. Nuovo et al, An Improved Technique for the In Situ Detection of DNA After Polymerase Chain Reaction Amplification, American Journal of Pathology, vol. 139, No. 6, Dec. 1991, pp. 1239-1244.
Kuo-Ping Chiu et al, Intracellular Amplification of Proviral DNA in Tissue Sections Using the Polymerase Chain Reaction, The Journal of Histochemistry and Cytochemistry, vol. 40, No. 3, pp. 333-341 (1992).
Paul Komminoth, M.D. et al, In Situ Polymerase Chain Reaction Detection of Viral DNA, Single-Copy Genes, and Gene Rearrangements in Cell Suspensions and Cytospins Diagnostic Molecular Pathology 1(2): pp. 85-97 (1992).
Ashley T. Haase et al, Amplification and Detection of Lentiviral DNA Inside Cells Proc. Natl. Acad. Sci. USA 87, pp. 4971-4975 (1990).
Janet Embretson et al, Analysis of Human Immunodeficiency Virus-Infected Tissues by Amplification and In Situ Hybridization Reveals Latent & Permissive Infections At Single-Cell Resolution Proc. Natl. Acad. Sci. USA 90, pp. 357-361 (1993).
John Findlay, Development of PRC for In Vitro Diagnostics (1992).
Materials received at the Tufts University Symposium on In Situ DNA Hybridization Mar. 1991, entitled "In Situ PCR, General Methods and Applications".
H. J. Wolfe et al, Verh. Dtsch. Ges. Path. 74, pp. 295-300 (1990).
O. Bagasra et al, Detection of Human Immunodeficiency Virus Type 1 Provirus in Mononuclear Cells by In Situ Polymerase Chain Reaction, New England Journal of Medicine, 326, #2, pp 1385-1391 (1992).
Letters to the Editor, Detection of HIV Provirus by In Situ Polymerase Chain Reaction, New England Journal of Medicine, 327, #21, pp. 1529-1530 (1992).
G. J. Nuovo, Determination of Human Papillomavirus Type by In Situ Hybridization Analysis: A Comparative Study with Southern Blot Hybridization and Polymerase Chain Reaction, The Journal of Histotechnology, 15, #2, pp. 99-104 (1992).
G. J. Nuovo et al, Detection of Human Papillomavirus DNA in Formalin-Fixed Tissues by In Situ Hybridization After Amplification by Polymerase Chain Reaction, American Journal of Pathology, 139, #4, pp. 847-854 (1991).
M. J. Embleton et al, In-Cell PCR From MRNA: Amplifying and Linking the Rearranged Immunoglobulin Heavy and Light Chain V-Genes Within Single Cells, Nucleic Acids Research, 20, #15, pp. 3831-3837 (1992).
Atwood John G.
Haff Lawrence A.
Beisner William H.
Grimes Edwin T.
The Perkin-Elmer Corporation
LandOfFree
In situ PCR amplification system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with In situ PCR amplification system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In situ PCR amplification system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1097088