In situ PCR amplification system

Details In situ PCR amplification system In situ PCR amplification system

1995-06-05

1996-06-18

Warden, Jill

Chemical apparatus and process disinfecting, deodorizing, preser

Control element responsive to a sensed operating condition

422 99, 422102, 359391, 359398, 356244, 211 41, B01L 900

Patent

active

055275109

ABSTRACT:
A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover over the sample, and a retaining assembly fastened to the slide and to the cover to retain and seal a reaction mixture against the sample during thermal cycling. The retaining assembly includes a rigid ring on a rim portion of the cover, a cross beam having spaced parallel rails joined by opposite flat ends, and a pair of clips which are pressed over the ends and opposite sides of the slide to fasten the cross beam and cover to the slide.

REFERENCES:
patent: 3029695 (1962-04-01), Wolf
patent: 3220300 (1965-11-01), von Huene
patent: 3233975 (1966-02-01), McCormick
patent: 3503665 (1970-03-01), Carter
patent: 3667088 (1972-06-01), Kurtz
patent: 3879106 (1975-04-01), McCormick
patent: 3883398 (1975-05-01), Ono
patent: 4384193 (1983-05-01), Kledzik et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4974952 (1990-12-01), Focht
patent: 4985206 (1991-01-01), Bowman et al.
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5062697 (1991-11-01), Mitchell
patent: 5281516 (1994-01-01), Stapleton et al.
Katherine A. Staskus et al, In Situ Amplification Of Visna Virus DNA In Tissue Sections Reveals A Reservoir Of Latently Infected Cells, Microbial Pathogenesis, 11, pp. 67-76 (1991).
Gerard J. Nuovo et al, An Improved Technique For The In Situ Detection Of DNA After Polymerase Chain Reaction Amplification, American Journal of Pathology, vol. 139, No. 6, Dec. 1991, pp. 1239-1244.
Kuo-Ping Chiu et al, Intracellular Amplification Of Proviral DNA In Tissue Sections Using The Polymerase Chain Reaction, The Journal of Histochemistry and Cytochmistry, vol. 40, No. 3, pp. 333-341 (1992).
Paul Komminoth, M. D. et al, In Situ Polymerase Chain Reaction Detection Of Vira DNA, Single-Copy Genes, And Gene Rearrangements In Cell Suspensions And Cytospi Diagnostic Molecular Pathology 1(2): pp. 85-97 (1992).
Ashley T. Haase et al, Amplification And Detection Of Lentiviral DNA Inside Cell Proc. Natl. Acad. Sci. USA 87, pp. 4971-4975 (1990).
Janet Embretson et al, Analysis Of Human Immunodeficiency Virus-Infected Tissues By Amplification And In Situ Hybridization Reveals Latent & Permissive Infection At Single-Cell Resolution Proc. Natl. Acad. Sci. USA 90, pp. 357-361 (1993).
John Findlay, Development Of PCR For In Vitro Diagnostics (1992).
Materials received at the tufts University Symposium on In Situ DNA Hybridization Mar. 1991, entitled "In Situ PCR, General Methods and Applications".
H. J. Wolfe et al, Verh. Dtsch. Ges. Path. 74, pp. 295-300 (1990).
O. Bagasra et al, Detection Of Human Immunodeficiency Virus Type 1 Provirus In Mononuclear Cells By In Situ Polymerase Chain Reaction, New England Journal Of Medicine, 326, #2, pp. 1385-1391 (1992).
Letters to the Editor, Detection Of HIV Provirus By In Situ Polymerase Chain Reaction, New England Journal of Medicine, 327, #21, pp. 1529-1530 (1992).
G. J. Nuovo, Determination Of Human Papillomavirus Type By In Situ Hybridization Analysis: A Comparative Study With Southern Blot Hybridization And Polymerase Chain Reaction, The Journal of Histotechnology, 15, #2, pp. 99-104 (1992).
G. J. Nuovo et al, Detection Of Human Papillomavirus DNA In Formalin-Fixed Tissue By In Situ Hybridization After Amplification By Polymerase Chain Reaction, American Journal of Pathology, 139, #4, pp. 847-854 (1991).
M. J. Embleton et al, In-Cell PCR From MRNA: Amplifying And Linking The Rearranc Immunoglobulin Heavy And Light Chain V-Genes Within Single Cells, Nucleic Acids Research, 20, #15, pp. 3831-3837 (1992).
Diagnostic Molecular Pathology, vol. 1, No. 2, Jun. 1992, New York, US pp. 85-97--Komminoth Et Al. `In Situ Polymerase Chain Reaction Detection of Viral DNA, Single-Copy Genes, and Gene Rearrangements in Cell Suspensions and Cytospin` p. 86, right column--p. 87, left column.

Affiliated with

Also associated with

Rating

In situ PCR amplification system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In situ PCR amplification system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In situ PCR amplification system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-221022