Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-03-22
1996-12-31
Rollins, John W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435123, C12Q 132, C12P 1702
Patent
active
055893481
ABSTRACT:
The invention relates to an improved enzymatic method for determining the concentration of an analyte in a sample body fluid. The improvement comprises a coupled enzyme reaction scheme comprising the in situ generation of reduced nicotinamide coenzyme, with either simultaneous or subsequent re-oxidation of coenzyme by an analyte substrate and substrate specific enzymes. The change in absorbance due to the reoxidation of coenzyme is proportional to the concentration of analyte in the reaction mixture.
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Kaufman Richard A.
Konopka John M.
Rosenfeld Henry J.
Sabo Janine E.
Hoffmann-La Roche Inc.
Johnston George W.
Rocha-Tramaloni Patricia S.
Rollins John W.
Semionow Raina
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