In situ hybridization to detect specific nucleic acid sequences

Details In situ hybridization to detect specific nucleic acid sequences In situ hybridization to detect specific nucleic acid sequences

1996-10-02

1999-03-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536243, 530300, C12Q 168, C07H 2104, C07K 500

Patent

active

058887334

ABSTRACT:
Methodologies for determining the presence of specific nucleic acid sequences in a sample of eucaryotic origin using in situ hybridization are described. The in situ hybridization is performed using a hybridization solution comprising at least one binding partner capable of hybridizing to the specific nucleic acid sequence to be determined so as to form hybrids and a hybrid destabilizing agent in an amount effective to decrease the melting temperature of hybrids formed between the nucleic acid and the binding partner so as to increase the specific binding and decrease the non-specific binding. The binding partner used is a polymeric strand of polymerized moieties having a non-cyclic backbone, the polymeric strand being capable of hybridizing to the nucleic acid sequence to be determined. The method is particularly suitable for diagnosing different human diseases such as bacterial and viral infections, genetic diseases and neoplastic disorders.

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