Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-04-07
1998-05-12
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 405, 435 4052, C12Q 168, A61K 944, G01N 130
Patent
active
057503400
ABSTRACT:
A fluorescence in situ hybridization (FISH) procedure and solutions is provided. The entire FISH procedure is fast (15 minutes or less), with the hybridization step occurring in 5 minutes or less. The entire FISH procedure with digoxygenin- or biotin-labeled probes takes approximately 30 minutes. A formamide-free solution (dextran sulfate and glycerol) is provided. An additional solution (10% dextran sulfate and 20% formamide) is also provided. Additional solutions are provided for mRNA in situ hybridization whereby the process takes less than 24 hours.
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Kim In C.
Sarto Gloria E.
Thompson Donald M.
Atzel Amy
Jones W. Gary
Kehl G. Dickson
Myers Jeffrey D.
Peacock Deborah A.
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