In situ hybridization method

Details In situ hybridization method In situ hybridization method

1991-09-04

1996-04-09

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, C12Q 168, C12N 1563

Patent

active

055060986

ABSTRACT:
A method of identifying the presence of a known target sequence in double-stranded DNA contained in a fixed cellular or subcellular biological structure. By adding a stable, reporter-labeled RecA/single-stranded probe complex to the structure, the target sequence can be effectively labeled by in situ hybridization, allowing the target sequence to be visualized histologically and microscopically or detected by in situ cytometry or cell sorting flow techniques.

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Weier et al., Chromosoma 100:371-376 (1991) "Two-color hybridization with high complexity . . . ".
Fan et al., P.N.A.S. 87:6223-6227 (1990) "Mappling Small DNA sequences by fluorescence . . . ".
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