In situ assay of amplified intracellular mRNA targets

Details In situ assay of amplified intracellular mRNA targets In situ assay of amplified intracellular mRNA targets

1994-03-22

1996-04-09

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435912, 435 9121, 435 5, 935 16, 935 78, C12Q 170, C12Q 168, C12P 1934

Patent

active

055061052

ABSTRACT:
In an in situ hybridization assay, cells are fixed under conditions which preserve essential morphology, followed by transcription-based nucleic acid amplification of intracellular mRNA targets. The amplified targets are visualized by hybridizing labelled probes to the target sequences. The assay thereby provides a convenient method for detecting specific gene expression associated with morphologic characteristics of diagnostic significance.

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