Improvement of electrophoretic element using polyacrylamide gel

Details Improvement of electrophoretic element using polyacrylamide gel Improvement of electrophoretic element using polyacrylamide gel

1986-09-03

1988-02-02

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041827, 2041828, C25D 1302, C25D 1308

Patent

active

047227770

ABSTRACT:
An element for electrophoresis suitably employable for analysis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic material support, an adhesive layer containing an inorganic oxide and a binder, a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a plastic material covering film. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

REFERENCES:
patent: 3875044 (1975-04-01), Renn et al.
patent: 3922432 (1975-11-01), Renn
patent: 3960499 (1976-06-01), White
patent: 4415428 (1983-11-01), Nochumson et al.

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