Improved process for the purfication of synthetic oligonucleotid

Details Improved process for the purfication of synthetic oligonucleotid Improved process for the purfication of synthetic oligonucleotid

1985-08-27

1991-03-05

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, 536127, C07H 1900, C07H 2100

Patent

active

049979272

ABSTRACT:
A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.

REFERENCES:
patent: 4430496 (1984-02-01), Abbott
Fieser and Fieser, Reagents for Organic Synthesis, vol. 1, pp. 1255-1256, 1967, J. Wiley and Sons, N.Y.
Handbook of Chemistry and Physics, 60th ed. R. C. Weast and M. J. Astle eds., CRC Press, Boca Raton, FL 1981, p. C-373.
Lehninger, Biochemistry, Worth Publishers Inc., New York, N.Y., 1975, p. 875.

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