Improved method for assaying proteolytic enzymes

Details Improved method for assaying proteolytic enzymes Improved method for assaying proteolytic enzymes

1986-08-15

1990-08-21

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 24, 436800, 548161, 548194, C12Q 138

Patent

active

049505939

ABSTRACT:
A reagent for proteolytic enzyme assays has the general formula ##STR1## where RCO-- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.

REFERENCES:
patent: 4268628 (1981-05-01), Klose et al.
patent: 4307188 (1981-12-01), White
patent: 4405711 (1983-09-01), Masuda et al.
"Heterocyclic Azo Dyes by Oxidative Coupling", Hunig et al., Angen. Chem. Int'l. Edit. vol. 1, No. 12 (1962) pp. 640-646.
"Spot Test Detection and Colorimetric Determination of Aromatic Amines and Imino Heteroaromatic Compounds with 3-Methyl-2-benzothiazolone Hydrazone", Sawicki et al., Analytical Chemistry, vol. 33, No. 6, May 1961, pp. 722-725.

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