Impregnated disk method for testing antifungal susceptibility

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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C12K 104

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active

041682068

ABSTRACT:
A method for testing the susceptibility of fungi to antifungal agents comprises inoculating a non-nutrient material, such as agar, with a fungus. A number of disks impregnated with various types and concentrations of antifungal agents and the proper nutrient medium for the type of fungus and antifungal agent tested is placed upon the non-nutrient medium inoculated with fungus. After an incubation period, the plates are read to determine the minimum inhibitory concentration of the particular antifungal agent for the particular fungus. The minimum fungicidal concentration can also be determined upon further incubation.

REFERENCES:
patent: 2904474 (1959-09-01), Forg
patent: 3107204 (1963-10-01), Brown et al.
patent: 3713985 (1973-01-01), Astle
Shadomy et al., Manual of Clinical Microbiology, 2nd. Ed., pp. 569.574 (1974).

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