Impaired dominant selectable marker sequence and intronic insert

Details Impaired dominant selectable marker sequence and intronic insert Impaired dominant selectable marker sequence and intronic insert

1995-06-07

1998-03-31

Ketter, James

Chemistry: molecular biology and microbiology

Vector, per se

536 231, 536 241, C12N 1585

Patent

active

057337799

ABSTRACT:
Disclosed herein are fully impaired consensus Kozak sequences which are most typically used with dominant selectable markers of transcriptional cassettes which are a part of an expression vector. These vectors are most typically utilized in the expression of proteins in mammalian expression systems. As defined, disclosed and claimed herein, a "fully impaired consensus Kozak" comprises the following sequence: ##STR1## where: "x" is a nucleotide selected from the group consisting of adenine (A), quanine (G), cytosine (C) or thymine (T)/uracil (U); "Py" is a pyrimidine nucleotide, ie C or T/U; "ATG" is a codon encoding for the amino acid methionine, the so-called "start" codon; and -3 and +1 are directional reference points vis-a-vis ATG, ie -3 is meant to indicate three nucleotides upstream of ATG and +1 is meant to indicate one nucleotide downstream of ATG. Dominant selectable markers further comprising artificial intronic insertion regions are further disclosed.

REFERENCES:
Yenofsky, 1990, Natl. Acad. Sci., 87: 3435-3439.
Abrams, 1989, J. Biol. Chem., 264: 14016-14021.
Kozak, 1989, J. Biol. Chem., 108: 229-241.
Kozak, 1986, Proc. Natl. Acad. Sci., 83: 2850-2854.
Kozak, 1987, Mol. Cell Biol., 7(10): 3438-3445.
Kozak, 1984, Mol. Cell Biol., 9(11): 5073-5084.
Niwa, 1991, Gene, 108: 193-200.
Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Colo. Springs/Harbor Press, Colo. Spring Harbor NY.
Hartman et al., 1988, Proc. Natl. Acad.Sci., 85: 8047-8051.

Affiliated with

Also associated with

Rating

Impaired dominant selectable marker sequence and intronic insert does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Impaired dominant selectable marker sequence and intronic insert, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Impaired dominant selectable marker sequence and intronic insert will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-51188