Drug – bio-affecting and body treating compositions – Conjugate or complex of monoclonal or polyclonal antibody,... – Conjugated to proteinaceous toxin or fragment thereof
Patent
1997-04-15
2000-11-14
Saunders, David
Drug, bio-affecting and body treating compositions
Conjugate or complex of monoclonal or polyclonal antibody,...
Conjugated to proteinaceous toxin or fragment thereof
4241341, 4241721, 514 12, 514 13, 530326, 530370, 5303873, 5303917, 5303919, A61K 39395, A61K 3800, A61K 3804, A61K 3578
Patent
active
06146631&
ABSTRACT:
The present invention provides purified and isolated polynucleotides encoding Type I ribosome-inactivating proteins (RIPS) and analogs of the RIPs having a cysteine available for disulfide bonding to targeting molecules. Vectors comprising the polynucleotides and host cells transformed with the vectors are also provided. The RIPs and RIP analogs are particularly suited for use as components of cytotoxic therapeutic agents of the invention which include gene fusion products and immunoconjugates. Cytotoxic therapeutic agents or immunotoxins according to the present invention may be used to selectively eliminate any cell type to which the RIP component is targeted by the specific binding capacity of the second component of the agent, and are suited for treatment of diseases where the elimination of a particular cell type is a goal, such as autoimmune disease, cancer and graft-versus-host disease.
REFERENCES:
patent: 4675382 (1987-06-01), Murphy
patent: 4769326 (1988-09-01), Rutter
patent: 4853871 (1989-08-01), Pantoliano et al.
patent: 4888415 (1989-12-01), Lambert et al.
patent: 4894443 (1990-01-01), Greenfield et al.
patent: 4925673 (1990-05-01), Steiner et al.
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5013653 (1991-05-01), Huston et al.
patent: 5028530 (1991-07-01), Lai et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5093475 (1992-03-01), Carroll et al.
patent: 5101025 (1992-03-01), Piatak, Jr. et al.
patent: 5169939 (1992-12-01), Gefter et al.
patent: 5376546 (1994-12-01), Bernhard et al.
patent: 5416202 (1995-05-01), Bernhard et al.
patent: 5621083 (1997-04-01), Better et al.
patent: 5624827 (1997-04-01), Rosenblum et al.
patent: 5631348 (1997-05-01), Rosenblum et al.
patent: 5744580 (1998-04-01), Better et al.
patent: 5756699 (1998-05-01), Better et al.
patent: 5837491 (1998-11-01), Better et al.
Better et al., Protein ant-CD5 ricin A chain immunoconjugates from bacterially produced Fab' and F(ab')2', Proc. Natl. Acad. Sci. USA 90:457-461, Jan. 1993.
Bacha, P. et al., J. Exp. Med., 167:612-622 (Feb. 1988) "Interleukin 2 Receptor-Targeted Cytotoxicity".
Batra, J.K. et al., J. Biol. Chem., 265 (25):15198-15202 (Sep. 1990) "Anti-Tac(Fv)-PE40, a Single Chain Antibody Pseudomonas Fusion Protein Directed at Interleukin 2 Receptor Bearing Cells".
Chaudhary, V.K. et al., Nature, 339:394-397 (Jun. 1989) "A Recombinant Immunotoxin Consisting of Two Antibody Variable Domains Fused to Pseudomonas Exotoxin".
Colombatti, M. et al., J. Immunol., 131 (6):3091-3095 (Dec. 1983) "Selective Killing of Target Cells By Antibody-Ricin A Chain or Antibody-Gelonin Hybrid Molecules: Comparison of Cytotoxic Potency and Use in Immunoselection Procedures".
Descotes, G. et al., J. Immunopharmac., 7 (4):455-463 (1985) "The Immunological Activity of Plant Toxins Used in the Preparation of Immunotoxins--II. The Immunodepressive Activity of Gelonin".
Kohr, W.J. et al., Abstr. T15, The Protein Society, 4th Symposium. Aug. 11-15, 1990, San Diego, California "The Amino Acid Sequence of Gelonin".
Montecucchi, P.C. et al., Int. J. Peptide Protein Res., 33:263-267 (1989) "N-terminal Sequence of Some Ribosome-Inactivating Proteins".
O'Hare, M. et al., FEBS, 273 (1,2):200-204 (Oct. 1990) "Cytotoxicity of a Recombinant Ricin-A-Chain Fusion Protein Containing a Proteolytically-Cleavable Spacer Sequence".
Sivam, G. et al., Cancer Research, 47:3169-3173 (Jun. 1987).
"Immunotoxins to a Human Melanoma-Associated Antigen: Comparison of Gelonin With Ricin and Other A Chain Conjugates".
Stirpe, F. et al., J. Biol. Chem., 255 (14):6947-6953 (Jul. 1980) "Gelonin, A New Inhibitor of Protein Synthesis, Nontoxic to Intact Cells".
Westby, M. et al., Bioconjugate Chem., 3 (5):375-381 (Sep. 1992) "Preparation and Characterization of Recombinant Proricin Containing an Alternative Protease-Sensitive Linker Sequence".
Williams, D.P. et al., Protein Engineering, 1 (6):493-498 (1987) "Diptheria Toxin Receptor Binding Domain Substitution with Interleukin-2: Genetic Construction and Properties of a Diphtheria Toxin-Related Interleukin-2 Fusion Protein".
Batra, J.K. et al., Mol. and Cell. Biology, 11:2200-2205 (Apr. 1991) "Single-Chain Immunotoxins Directed at the Human Transferrin Receptor Containing Psuedomonas Exotoxin A or Diphtheria Toxin: Anti-TFR(Fv)-PE40 and DT388-Anti-TFR(Fv)".
Queen, C. et al., Proc. Natl. Acad. Sci. (USA), 86:10029-10033 (Dec. 1989) "A Humanized Antibody that Binds to the Interleukin 2 Receptor".
Nolan, P.A. et al., Gene, 134:223-227 (1993) "Cloning and Expression of a Gene Encoding Gelonin, A Ribosome-Inactivating Protein from Gelonium Multiflorum".
Ebert, R.F. et al. , Bioconjugate Chem., 1:331-336 (Feb. 1990) "Immunotoxin Construction with a Ribosome-Inactivating Protein from Barley".
Kernan, N.A. et al., J. Immunol., 133 (1):137-146 (Jul. 1984) "Specific Inhibition of In Vitro Lymphocyte Transformation By An Anti-Pan T Cell (gp67) Ricin A Chain Immunotoxin".
Kong, K. et al., American College of Toxicology, Oct. (1992) San Francisco, CA "Subacute Intranvenous Toxicity of a Ribosomal Inhibitory Protein rGelonin in Rats"(Poster).
Kohn, F.R. et al., Int. J. Immunopharmac., 15 (8):871-878 (Nov. 1993) "Efficacy of Anti-CD5 F(AB').sub.2 and Fab' Immunoconjugates In Human Peripheral Blood Lymphocyte-Reconstituted Severe Combined Immunodeficient Mice".
Better, M. et al., Proc. Natl. Acad. Sci. USA, 90:457-461 (Jan. 1993) "Potent Anti-CD5 Ricin A Immunoconjugates from Bacterially Produced Fab' and F(ab').sub.2 ".
Better, M. et al., J. Biol. Chem., 267 (23):16712-16718 (Aug. 1992) "Activity of Recombinant Mitogillin and Mitogillin Immunoconjugates".
Better, M. et al., J. Biol. Chem., 269 (13):9644-9650 (Apr. 1994) "Gelonin Analogs with Engineered Cysteine Residues Form Antibody Immunoconjugates with Unique Properties".
Lambert, J.M. et al., J. Biol. Chem., 260 (22):12035-12041 (Oct. 1985) "Purified Immunotoxins That Are Reactive with Human Lymphoid Cells".
Better, M. et al., 3rd Int'l Symposium on Immunotoxins, Jun. 19-21 (1992) Orlando, FL "Generation of Potent Immunoconjugates from Microbially Produced Fab', F(ab').sub.2, and Recombinant Ribosome Inactivating Proteins" (Poster).
Brown, D. et al., The Washington Post, A1 and A4, Aug. 21, 1992 "Customizing Lab Mice".
Strockbine, N.A. et al., J. Bact., 170:1116-1122 (1988) "Cloning and Sequencing of the Genes for Shiga Toxin from Shigella dysenteria Type 1".
Tolan, D.R. et al., J. Biol. Chem., 259 (2):1127-1131 (1984) "The Complete Nucleotide Sequence for Rabbit Muscle Aldolase A Messenger RNA".
Reichmann, L. et al., Nature, 332:323-327 (Mar. 1988) "Reshaping Human Antibodies for Therapy".
Royston, I. et al., J. Immunol., 125 (2):725-731 (Aug. 1980) "Human T Cell Antigens Defined By Monoclonal Antibodies: The 65,000-Dalton Antigen of T Cells (T65) Is Also Found On Chronic Lymphocytic Leukemia Cells Bearing Surface Immunoglobulin".
Stirpe, F. et al., FEBS, 195 (1, 2):1-8 (Jan. 1986) "Ribosome-inactivating proteins up to date".
Ready, M. et al., J. Biol. Chem., 259 (24):15252-15256 (Dec. 1984) "Ricin-like Plant Toxins Are Evoluntionarily Related to Single-chain Ribosome-inhibiting Proteins from Phytolacca".
Katzin, B.J. et al., Protein, 10:251-259 (Jun. 1991) "Structure of Ricin A-Chain at 2.5 .ANG.".
Myers, E.W. et al., Cabios Communications, 4 (1):11-17 (1988) "Optimal alignments in linear space".
Islam, M.R. et al., Agricultural Biological Chem., 54 (5):1343-1345 (May 1990) "Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Luffa cylindrica".
Chow, T. et al., J. Biol. Chem., 265:8670-8674 (May 1990) "Isolation and DNA Sequence of a Gene Encoding .alpha.-Trichosanthin, a Type I Ribosome-inactivating Protein".
Ho, W.K. et al., Biochem. Biophys. Acta, 1088:311-314 (Feb. 1991) "Cloning of the cDNA of .alpha.-momorcharin: a ribosome inactivating protein".
Habuka, N. et al., J. Biol. Chem., 264 (12):6629-6637 (Apr. 1989) "Amino Acid Sequence of Mirabilis Antiviral Protein, Total Synthesis of Its Gene and Expression in Escherichia coli".
Kung, S.-S. et al., Agricultural Biological Chem., 54 (12):3301-3318 (Dec. 1
Better Marc D.
Carroll Stephen F.
Studnicka Gary M.
Saunders David
Tung Mary Beth
Xoma Corporation
LandOfFree
Immunotoxins comprising ribosome-inactivating proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunotoxins comprising ribosome-inactivating proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunotoxins comprising ribosome-inactivating proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2062325