Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1994-04-08
1997-12-16
Nucker, Christine M.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
436518, 435 71, 530328, 530350, 530827, G01N 33567
Patent
active
056984480
ABSTRACT:
Purified immunosuppressive drug binding proteins (immunophilins) of molecular mass 2.4-3.0 kDa, 4.5 kDa, 34-37 kDa, 50-54 kDa, 80-100 kDa, and greater than about 120 kDa are described. The 34-37 kDa immunophilin specifically binds FK-506 and rapamycin. The 50-54 kDa immunophilin specifically binds FK-506, rapamycin and cyclosporine A, but with binding site distinctions. The 50-54 kDa immunophilin is devoid of significant rotomase activity, but inhibits cAMP-activated protein kinase activity. The amino acid composition, and the sequences of a dodecameric amino acid C-terminus partial sequence and of two heptameric internal partial amino acid sequences, of the 50-54 kDa immunophilin are described; the deduced molecular weight is 52,171. Recombinant about 52 kDa immunophilin is also described. These novel immunophilins can be used as reagents for the detection, quantification and capture of immunosuppressive drugs and their biologically active metabolites, derivatives and analogues in fluid samples, and for the capture of potential immunosuppressive drugs from microbial extracts or culture media or from mammalian body fluids and tissues.
REFERENCES:
patent: 4668640 (1987-05-01), Wang et al.
patent: 4722999 (1988-02-01), Handschumacher et al.
patent: 4727035 (1988-02-01), Mahoney
patent: 5109112 (1992-04-01), Siekierka et al.
patent: 5196352 (1993-03-01), Siekierka et al.
Harding et al. J. Biol. Chem., 261:8547 (1986).
Agarwal et al. Transplantation, 42:627 (1986).
Takahashi et al. Nature, 337:473 (1989).
Steiner et al. FASEB J., 2:9025A, p. 1850, (1988).
Merker et al. J. Immunol., 132:3064 (1984).
Donnelly et al. Ther. Drug Monitor, 11:696 (1989).
Quesniaux et al. Clin. Chem., 33:1, pp. 32-37, (1987).
Rosborough et al. Transplant Proc., 23:2890 (1991).
Tai et al. Biochem., 25:5269 (1986).
Fretz et al. J. Am. Chem. Soc., 113:1409 (1991).
Koletsky et al. J. Immunol., 137:3, pp. 1054-1059, (1986).
Siekierka et al. Nature, 341:755 (1989).
Harding et al. Nature, 341:758 (1989).
Palaszynski et al. Clin. Biochem., 24:63 (1991).
Russell et al. Ther. Drug Monitor, 13:32 (1991).
Donnelly et al. Clin. Biochem., 24:71 (1991).
Donnelly et al. Transplant. Proc., 23:2886 (1991).
Nucker Christine M.
Stucker Jeffrey
LandOfFree
Immunosuppressive drug binding proteins and use does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunosuppressive drug binding proteins and use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunosuppressive drug binding proteins and use will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-205204