Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-02-12
1990-10-16
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 435805, 436501, 436514, 436518, 436530, 436541, 436810, 436815, 436816, G01N 33535, G01N 33539, G01N 33543, G01N 33558
Patent
active
049634682
ABSTRACT:
A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a piece of bibulous material capable of being traversed in at least one direction by the test solution through capillary action. The bibulous material contains a first receptor capable of binding to said conjugate. The first receptor is non-diffusively bound to a situs on the bibulous material separate from the contact portion. The bibulous material further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the bibulous material. At least a portion of the test solution is allowed to traverse the bibulous material by capillary action and thereby contact the situs. The situs is examined for the presence of the conjugate. To this end, the situs can be exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.
REFERENCES:
patent: 3990850 (1976-11-01), Friedman et al.
patent: 4055394 (1977-10-01), Friedman et al.
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4160008 (1979-07-01), Fenocketti et al.
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4189304 (1980-02-01), Adams, Jr. et al.
patent: 4205058 (1980-05-01), Wagner et al.
patent: 4327073 (1982-04-01), Huang
patent: 4366241 (1982-12-01), Tom et al.
patent: 4384958 (1983-05-01), Wisebaker et al.
patent: 4434236 (1984-02-01), Freytag
patent: 4438067 (1984-03-01), Siddiqi
patent: 4666863 (1987-05-01), Edwards et al.
patent: 4740468 (1988-04-01), Weng et al.
patent: 4806311 (1989-02-01), Greenquist
Kepplinger Esther L.
Leitereg Theodore J.
Saunders David A.
Swiss Gerald F.
Syntex (U.S.A.) Inc.
LandOfFree
Immunoseparating strip does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunoseparating strip, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoseparating strip will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-849456