Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-07-05
1992-02-04
Saunders, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 435 793, 435970, 435975, 436501, 436514, 436518, 436530, 436541, 436810, 436815, 436816, G01N 33538, G01N 33543, G01N 33548
Patent
active
050859889
ABSTRACT:
A method and device for determining the presence of an analyte in a sample suspected of containing the analyte are disclosed. The method involves contracting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The strip contains a first receptor capable of binding to the conjugate. The first receptor is non-diffusively bound to a situs on the strip separate from the contact portion of the strip. The strip further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the strip. At least a portion of the test solution is allowed to traverse the strip by capillary action and thereby contact the situs. The strip is exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.
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Leitereg Theodore J.
Saunders David A.
Syntex (U.S.A.) Inc.
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