Immunoselection system for cell elution

Details Immunoselection system for cell elution Immunoselection system for cell elution

1996-02-12

1998-06-30

Feisee, Lila

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 436518, G01N 3353, G01N 33567, G01N 33543

Patent

active

057732248

ABSTRACT:
The recovery of immunoselected cells is improved by using a specific affinity elution system utilizing members of an immobilized ligand/antiligand pair, with soluble ligand used for elution. In one embodiment, Immobilized heparin adsorbent is coated with biotinylated antithrombin III which is then crosslinked with avidin, thereby greatly increasing the binding affinity of antithrombin III for heparin. The resulting adsorbent effectively captures biotin-labeled target cells and allow facile elution of immunoselected cells with soluble heparin. The cell separation system is capable of both positive and negative selection.

REFERENCES:
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4298685 (1981-11-01), Parikh et al.
patent: 4468470 (1984-08-01), Aalberse
patent: 5518882 (1996-05-01), Lund et al.

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