Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1987-09-18
1989-03-14
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
435 7, 435 36, 436534, 436547, 436825, G01N 33546, G01N 33547, G01N 33569
Patent
active
048124141
ABSTRACT:
A reagent useful in the determination of an immunoreactive species comprises water-insoluble particles having tracer molecules associated therewith. Bound to the outer surface of the particles are: (i) receptor molecules which are reactive with the species, and (ii) molecules of a protein having a pI less than about 6, and which protein is not reactive with either the species or the receptor molecules. The weight ratio of the receptor molecules to the low pI protein molecules is from about 100:1 to about 1:10. This reagent is useful in agglutination and other immunological reactions. A method of preparing the reagent includes providing a suspension of the particles and contacting them with the receptor molecules and low pI protein so as to attach both to the particles. The protein is present in the suspension in an amount such that substantially all of it is attached to the particles. The reagent thus prepared can be used, for example, in agglutination assays for the determination of a multivalent immunoreactive species, such as Streptococcus A antigen.
REFERENCES:
patent: 4307190 (1981-12-01), Masson
patent: 4459361 (1984-07-01), Gefter
patent: 4560504 (1985-12-01), Arnold
patent: 4591571 (1986-05-01), Kuboyama et al.
patent: 4618576 (1986-10-01), Rosenstein
patent: 4656144 (1987-04-01), Hosaka
patent: 4677080 (1987-06-01), Finkelstein
patent: 4713350 (1987-12-01), Siegel
L. F. Fieser et al., "Advanced Organic Chemistry", p. 1048, Reinhold, New York, 1961.
Johnson et al., Gene. Anal. Techn. 1 (1984) pp. 3-8.
Snyder Brian A.
Warren, III Harold C.
Eastman Kodak Company
Marantz Sidney
Tucker J. Lanny
LandOfFree
Immunoreactive reagent particles having tracer, receptor molecul does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunoreactive reagent particles having tracer, receptor molecul, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoreactive reagent particles having tracer, receptor molecul will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-893594