Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...
Patent
1992-11-20
1995-07-18
Draper, Garnette D.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Amino acid sequence disclosed in whole or in part; or...
530350, 4241901, 4242401, A61K 3900, A61K 3902, A61K 3700, A01N 6300
Patent
active
054339456
ABSTRACT:
A new method is described for the preparation of a safe, immunogenic and efficacious vaccine for protection against the disease pertussis. In development of this vaccine, specific functional sites of pertussis toxin have been identified, and using this information, defined mutant holotoxins have been produced by site directed mutagenesis of the toxin gene. A number of these holotoxin analogues are detoxified, retain an immunodominant S1 epitope, are immunogenic and are protective in the standard pertussis vaccine potency test in mice.
REFERENCES:
Arico et al. (Jun. 1987) J. of Bacteriology, vol. 169 (6): pp. 2847-2853.
Locht et al. (1986) Science, vol. 232:1258-1264.
Locht et al. (1986) Nucleic Acids Research vol. 14(8):3251-3261.
Nicosia et al. (1986) PNAS, vol. 83: pp. 4631-4635.
Isberg et al. (1985) Curr. Top. Microbiol. Immunol., vol. 118: pp. 1-11.
Moss et al., J. Biol. Chem. 258, 11879 [1983].
Locht and Keith, Science 232, 1258 [1986].
Carrol & Collier, Proc. Nat. Acad. Sci., U.S.A, 81, 3307 [1984].
Carroll & Collier, J. Biol. Chem., 262, 8707 [1987].
Nogimori et al., Biochem., 25, 1355 [1986 ].
Armstrong & Peppler, Infec. Immun., 55, 1294 [1987].
Tweten et al., J. Biol. Chem., 260, 10392 [1984].
Douglas & Collier, J. Bacteriol., 169, 4967, [1987].
Locht et al., Infect. Immun., 55, 2546, [1987].
Black et al., Ann. Sclavo, 175, [1986].
Black & Falkow, Infect. Immun., 55, 2465, [1987].
Burns et al., Infect. Immun., 55, 24, [1987].
Watkins et al., J. Biol. Chem., 260, 13478, [1985].
Ditta et al., Plasma, 13, 149, [1985].
Kahn et al., Methods in Enzymology, 68, 278, [1979]*.
Nicosia et al., Proc. Nat. Acad. Sci., U.S.A., 83, 4631 [1986].
Arico & Rappuoli, J. Bacteriol., 169, 2849, [1987].
Gene, vol. 50, 1986, pp. 133-140; Elsevier; S. Stibitz et al.: "The Construction of a cloning vector designed for gene replacement in Bordetella pertussis".
Biochemistry, vol. 21, 1982, pp. 5516-5522, American Chemical Society; M. Tamura et al.: "Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B mode"*.
Chemical Abstracts, vol. 105, No. 19, Nov. 10, 1986, ref. No. 166239w: Columbus, Ohio, US.
J. Reiser et al.: "Expression in Escherchia coli of a Bordetella pertussis toxin-coding region" & Vaccines 86, New Approaches Immun. (Proc. Conf.) 1985 (Pub. 1986), 235-8.
Infection and Immunity, vol. 56, No. 8, Aug. 1988, pp. 1934-1941; J. T. Barbieri et al.: "ADP-ribosyl transferase mutations in the catalytic S-1 subunit of pertussis toxin" Science, vol. 242, No. 4875, 1988, pp. 72-74.
Infection and Immunity, vol. 55, No. 4, Apr. 1987, pp. 963-967; A. Nicosia et al.: "Expression and immunological properties of the five subunits of pertussis toxin".
Burnette et al; "Pertussis Toxin S1 Mutant with Reduced Enzyme Activity and a Conserved Protective Epitope" Science--vol. 242, No. 487, 1988, pp. 72-74.
EP-A-0 145 486 (Glaxo Group Ltd.) p. 4, lines 12-27; p. 6, line 22, p. 9, line 12; example 2.
EP-A-0087 735 (Takeda Chemical Industries) p. 7, line 15-24.
Chemical Abstracts, vol. 106 , No. 11, 16th Mar. 1987, p. 163, abstract no. 79657h, Columbus, Ohio, US.
J. M. Keith et al.: "Pertussis toxin gene cloning and expression of protective antigen", & US-A-843 727 (United States Dept. of Health and Human Services) 20 Jun. 1986 *Abstract*.
Chemical Abstracts, vol. 106, No. 5, 2nd Feb. 1987, p. 120, Abstract No. 28346y, Columbus, Ohio, US.
R. M. Brownlie et al.: "Complementation of mutations in Escherichia coli and Bordetella pertussis by B. pertussis DNA cloned in a broad-host-range cosmid vector", & J. Gen. Michrobiol. 1986, 132(11), 3221-9 *Abstract*.
Burnette et al., Science, 1988; 242, 72-74.
Pizza et al., Science, 1989, 246:497-499.
Pizza et al., Bacterial Protein Toxins, 1990, 507-518.
Brown et al., Dev. Biol. Stand., 1991, 73:63-73.
Boux Heather A.
Cockle Stephen A.
Klein Michel H.
Loosmore Sheena M.
Zealey Gavin R.
Connaught Laboratories Limited
Draper Garnette D.
Wang Gian P.
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