Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Patent
1995-02-07
1999-12-28
Priebe, Scott D.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
536 231, 536 245, 536 241, 435 691, 4353201, 435325, 435455, 435458, A61K 4800, C12N 1500
Patent
active
060082003
ABSTRACT:
Oligonucleotides containing unthylated CpG dinucleotides and therapeutic utilities based on their ability to stimulate an immune response in a subject are disclosed. Also disclosed are therapies for treating diseases associated with immune system activation that are initiated by unthylated CpG dinucleotides in a subject comprising administering to the subject oligonucleotides that do not contain unmethylated CpG sequences (i.e. methylated CpG sequences or no CpG sequence) to outcompete unmethylated CpG nucleic acids for binding. Further disclosed are methylated CpG containing dinucleotides for use antisense therapies or as in vivo hybridization probes, and immunoinhibitory oligonucleotides for use as antiviral therapeutics.
REFERENCES:
patent: 4956296 (1990-09-01), Fahnestock
patent: 5234811 (1993-08-01), Beutler et al.
patent: 5585479 (1996-12-01), Holze et al.
patent: 5663153 (1997-09-01), Hutcherson et al.
patent: 5723335 (1998-03-01), Hutcherson et al.
patent: 5786189 (1998-07-01), Locht et al.
Ren jun et al., HCAPLUS Database, AN: 198874, Abstract, 1994.
Anfossi et al., HCAPLUS Database, AN: 475562, Abstract, 1989.
Stull et al., Pharmaceutical Res., vol. 12, 4:465-483, 1995.
Mastranjelo et al., Seminars in Oncology, vol. 23, 1:4-21, 1996.
Robert Whalen, Emerging Infectious Disease, vol. 2, 3:168-175, 1996.
Etlinger, Immunology Today, vol. 13, 2:52-55, 1992.
Sato et al., Science, vol. 273, 1996 : 352-354.
Crystal, Science, vol. 270, pp. 404-410, 1995.
Stein, C.A. et al. Oligodeoxynucleotides as inhibitors of gene expression: a review. Cancer Research 48:2659-2668, May 15, 1988.
Wu, G.Y. et al. Receptor-mediated gene delivery and expression in vivo. J. Biological Chemistry 263:14621-14624, Oct. 15, 1988.
Tanaka, T. et al. An antisense oligonucleotide comp;ementary to a sequence in IG2b germline transcripts, stimulates B cell DNA synthesis, and inhibits immunoglobulin secretion. J. Exp. Med. 175:597-607, Feb. 1, 1992.
Tokunaga, et al., J Cancer Res, (Gann 1988), 79:682.
Blaxter et al., Genes expressed in Brugia malayi infective third stage larvae, Molecular and Biochemical Parasitology, 77:77-93 (Apr. 1996).
Fox , R.I., Mechanism of action of hydroxychloroquine as anantirheumatic drug, Chemical Abstracts 120:15, Abstract No.182630 (Apr. 29, 1994).
Mottram et al., A novel CDC2-related protein kinase from leishmania mexicana,LmmCRK1, is post-translationally regulated during the life cycle, J. Biol. Chem. 268:28 21044-21052 (Oct. 1993).
Schnell et al., Identification and characterization of a Saccharomyces cerevisiae gene (PAR1) conferring resistance to iron chelators, Eur. J. Biochem., 200:487-493.
Wallace et al., Oligonucleotide probes for the screening of recombinant DNA libaries, Methods in Enzymology, 152:432-442 (1987).
Azuma, I., "Biochemical and Immunological Studies on Cellular Components of Tubercle Bacilli", Kekkaku 67(9):45-55 (1992).
Kataoka, T. et al., "Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG", Jpn. J. Cancer Res. 83:244-247 (Mar. 1992).
Kimura, Y., et al., "Binding of Oligoguanylate to Scavenger Receptors Is Required for Oligonucleotides to Augment NK Cell Activity and Induce IFN", J. Biochem. 116(5):991-994 (1994).
Tokunaga, T., et al., "Synthetic Oligonucleotides with Particular Base Sequences from the cDNA Encoding Proteins of Mycobacterium bovis BCG Induce Interferons and Activate Natural Killer Cells", Microbiol. Immunol. 36(1):55-66 (1992).
Yamamoto, S. et al., "DNA from Bacteria, but Not from Vertebrates, Induces Interferons, Activates Natural Killer Cells and Inhibits Tumor Growth", Microbiol. Immunol. 36(9):983-997 (1992).
Yamamoto, S., "Mode of Action of Oligonucleotide Fraction Extracted From Mycobacterium bovis BCG", Kekkaku 69(9):29-32 (1994).
Yamamoto, T. et al., "Ability of Oligonucleotides with Certain Palindromes to Induce Interferon Production and Augment Natural Killer Cell Activity Is Associated with Their Base Length", Antisense Res. and Devel. 4:119-123 (1994).
Yamamoto, T. et al., "Lipofection of Synthetic Oligodeoxyribonucleotide Having a Palindromic Sequence of AACGTT to Murine Splenocytes Enhances Interferon Production and Natural Killer Activity", Microbiol. Immunol. 38(10):831-836 (1994).
Yamamoto, T. et al., "Synthetic Oligonucleotides with Certain Palindromes Stimulate Interferon Production of Human Peripheral Blood Lymphocytes in vitro", Jpn. J. Cancer Res. 85:775-779 (1994).
International Search Report for PCT/US95/01570 (Jul. 4, 1995).
Yamamoto, Toshiko et al., "Ability of Oligonucleotides with Certain Palindromes to Induce Interferon Production and Augment Natural Killer Cell Activity is Associated with their Base Length", Antisense Research and Development, (1994), vol. 4, pp. 119-122.
Azad, Raana F. et al., "Antivoral Activity of a Phosphorothioate Oligonucleotide Complementary to RNA of the Human Cytomegalovirus Major Immediate-Early Region", Antimicrobial Agents and Chemotherapy, (Sep. 1993), vol. 37, pp. 1945-1954.
Messina et al., "The Influence of DNA Structure on the in vitro Stimulation of Murine Lymphocytes by Natural and Synthetic Polynucleotide Antigens", Cellular Immunology, (1993), vol. 147, pp. 148-157.
Branda et al., "Immune Stimulation by an Antisense Oligomer Complementary to the rev gene of HIV-1", Biochemical Pharmacology, (1993), vol. 45, No. 10, pp. 2037-2043.
Kuramoto et al., "Oligonucleotide Sequences Required for Natural Killer Cell Activation", Jpn. J. Cancer Res., (Nov. 1992), vol. 83, pp. 1128-1131.
Yamamoto et al., "Unique Palindromic Sequences in Synthetic Oliognucleotides are Required to Induce INF and Augment INF-Mediated NAtural Killer Activity", The Journal of Immunology, (Jun. 15, 1992), vol. 148, No. 12, pp. 4072-4076.
Kataoka, Tetsuro et al., "Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG", Japan J. Cancer Res., (Mar. 1992), vol. 83, pp. 244-247.
Tanaka et al., "An Antisense Oligonucleotide Complementary to a Sequence in I.gamma.2b Increases .gamma.2b Germline Transcripts, Stimulates B Cell DNA Synthesis, and Inhibits Immunoglobulin Secretion", The Journal of Experimental Medicine, (Feb. 1992), vol. 175, pp. 597-607.
Tokunaga, Tohru et al., "Synthetic Oligonucleotides with Particular Base Sequences from the cDNA encoding Proteins of Mycobacterium bovis BCG Induce Interferons and Activate Natural Killer Cells", Microbiol. Immunol., (1992), vol. 36(1), pp. 55-66.
Yamamoto, Saburop et al., "DNA from Bacteria, but not from Vertebrates, Induces Interferons, Activates Natural Killer Cells and Inhibits Tumor Growth", Microbiol. Immunol., (1992), vol. 36 (9), pp. 983-997.
Messina et al., "Stimulation of in vitro Murine Lymphocyte Proliferation by Bacterial DNA", The Journal of Immunology, (Sep. 15, 1991), vol. 147, No. 6, pp. 1759-1764.
Tokunaga et al., "A Synthetic Single-Stranded DNA, Ply (dG, dC), Induces Interferon .alpha./.beta. and -.gamma., Augments Natural Killer Activity and Suppresses Tumor Growth", Jpn. J. Cancer Res. (Gann), (Jun. 1988), vol. 79, pp. 682-686.
Nguyen Dave Trong
Priebe Scott D.
University of Iowa Research Foundation
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