Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-01-24
1995-12-19
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 436500, 436518, 436531, 436817, 436822, G01N 33545, G01N 33577
Patent
active
054767709
ABSTRACT:
The invention relates to a process for the immunometric determination of an antigen or hapten.
According to this process, contacting takes place (FIG. 1A) between the antigen or hapten (3) to be determined and the antibodies (2) fixed to a solid phase in order to immunologically bond the antigen or hapten with the antibody. This is followed (FIG. 1B) by immobilizing the antigen or hapten (3) by a covalent bond (4) to the solid phase (1) whilst releasing its epitope (FIG. 1C). This is followed by the contacting thereof (FIG. 1D) with labelled antibodies (5) and determination takes place (FIG. 1E) of the quantity of fixed labelled antibodies in order to deduce therefrom the initial hapten or antigen concentration.
As a result of this stage of immobilizing and releasing the epitope, a high sensitivity is obtained using a single antibody.
REFERENCES:
patent: 4376110 (1983-03-01), David et al.
patent: 4703001 (1988-10-01), Vodian et al.
patent: 4794090 (1988-12-01), Parham et al.
patent: 5236830 (1993-08-01), Ishikawa
Creighton, T. E. Protein Function. IRL Press at Oxford University Press, 1989. pp. 14-16.
Harlow, E. Antibodies: A Laboratory Manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 1988. pp. 26-28.
Pradelles, P., et al. "Immunometric Assay of Low Molecular Weight Haptens Containing Primary Amino Groups". Analytical Chemistry, 66:16-22, 1994.
Ikegaki, N., et al. "Gluteraldehyde fixation of the primary antibody-antigen complex on nitrocellulose paper increases the overall sensitivity of immunoblot assay." Journal of Immunological Methods, 124:205-210, 1989.
Ceperley Mary E.
Commissariat a l''Energie Atomique
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