Immunometric assays using monoclonal antibodies

Chemistry: analytical and immunological testing – Involving iga – igd – ige – or igm

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435 7, 436548, 436529, 436540, G01N 3354, G01N 3356

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active

043761100

ABSTRACT:
"Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies. One monoclonal antibody is presented in a soluble labeled form and a second monoclonal antibody is presented bound to a solid carrier; the soluble and bound monoclonal antibodies may be the products of either the same or different cell lines. Each monoclonal antibody has an affinity for the antigenic substances of at least about 10.sup.8 liters/mole.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs
patent: 3791932 (1974-02-01), Schuurs
patent: 3867517 (1975-02-01), Ling
patent: 4016043 (1977-04-01), Schuurs
patent: 4098876 (1978-07-01), Piasio
A. C. Cuello et al., Proc. Natl. Acad. Sci. U.S.A., vol. 76(7), 3532-3536 (Jul. 1979).

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