Immunometric assays for tumor necrosis factor-alpha and methods

Details Immunometric assays for tumor necrosis factor-alpha and methods Immunometric assays for tumor necrosis factor-alpha and methods

1988-12-01

1993-06-29

Lacey, David L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 794, 43524027, 530351, 53038823, 436548, G01N 3353, C07K 1528, C07K 1300, C12N 512

Patent

active

052233952

ABSTRACT:
An immunometric assay is disclosed for biologically active tumor necrosis factor in a biological sample comprising, forming a complex of a first labeled monoclonal antibody, biologically active TNF, and a second monoclonal antibody which can be bound to an insoluble substrate and detecting the amount of label associated with the complex. The assay is characterized by employing first and second monoclonal antibodies which react with the same epitopic site on TNF-alpha monomer. A method is also disclosed for blocking accelerated TNF degradation in a non-preserved biological sample, such as blood, comprising contacting the biological sample with EDTA, luminol, or a combination thereof.

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