Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...
Reexamination Certificate
2006-08-01
2006-08-01
Navarro, Mark (Department: 1645)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Amino acid sequence disclosed in whole or in part; or...
C424S190100, C424S234100, C530S300000, C530S350000, C530S825000
Reexamination Certificate
active
07083792
ABSTRACT:
Various immunologically active proteins fromBorrelia burgdorferihave been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from aB. burgdorferigene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. TheBorrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).
REFERENCES:
patent: 4888276 (1989-12-01), Shelburne
patent: 5620862 (1997-04-01), Padula
patent: 6183755 (2001-02-01), Motz et al.
patent: 6248538 (2001-06-01), Motz et al.
Antibodies, A Laboratory Manual, Harlow and Lane (ed),(1988),342, 343, 560-562, 5.
Barbour, Alan , et al., “Lyme Disease Spirochetes and Ixodid Tick Spirochetes Share a Common Surface Antigenic Determinant Defined by a Monoclonal Antibody”,Infection and Immunity, 41, (Aug. 1983),795-804.
Barbour, A. , “Plasmid Analysis of Borrelia Burgdorferi, the Lyme Disease Agent”,Journal of Clinical Microbiology, 26, (Mar. 1988),475-478.
Berzofsky, Jay , “Intrinsic and Extrinsic Factors in Protein Antigenic Structure”,Science, 229, (1985),932-940.
Bowie, et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions”,Science, 247, (Mar. 1990),1306-1310.
Coleman, James L., et al., “Identification and Characterization of an Endoflageliar Antigen of Borrelia burgdorferi”,The American Society for Clinical Investigation, Inc., The State of New York Department of Health, vol. 84,(Jul., 1989),322-330.
Fuchs, R. , et al., “Molecular analysis and expression of a Borrelia burgdorferi gene encoding a 22kDa protein (pC) inEscherichia coli”, Molecular Microbiology 6(4), (1992),503-509.
Gabmann, G.S. , et al., “N-terminal amino acid sequence of the Borrelia burgdorferi flagellin”,FEMS Microbiology Letters 60, Federation of European Microbiological Societies FEM 03611,(1989),101-105.
Gassmann, G. , et al., “Nucleotide Sequence of a Gene Encoding the Borrelia burgdorferi flagellin”,Nucleic Acids Research, 17, (1989),3590.
Hansen, K , et al., “Measurement of Antibodies to the Borrelia burgdorferi Flagellum Improves Serodiagnosis in Lyme Disease”,Journal of Clinical Microbiology, 26, (Feb. 1988),338-346.
Harlow, Ed , et al., “Antibodies—A Laboratory Manual”,Cold Spring Harbor Laboratory, (1988),553-612.
Hinnebusch, J. , et al., “Linear- and Circular-Plasmid Copy Numbers in Borrelia Burgdorferi”,Journal of Bacteriology, 174, (Aug. 1992),5251-5257.
Hopp, et al., “Prediction of Protein Antigenic Determinants from Amino Acid Sequences”,Proceedings of National Academy of Sciences, USA 78, (1981),3824-3828.
Hyde, F , et al., “Detection of Antigens in Urine of Mice and Humans Infected with Borrelia burgdorferi, Etiologic Agent of Lyme Disease”,Journal of Clinical Microbiology, 27, (1989),58-61.
Kumar, et al.,PNAS, 87, (Feb. 1990),1337-1341.
Lee, Cheng C., et al., “Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase”,Science, vol. 239, (Mar., 1988),1288-1291.
Luft, Benjamin J., et al., “Biochemical and Immunological Characterization of the Surface Proteins of Borrelia burgdorferi”,Infection and Immunity, Department of Medicine, State University of New York ant Stony Brook, vol. 57, No. 11,(Nov., 1989),3637-3645.
Pallesen, Lars , et al., “Cloning and Sequencing of a Treponema pallidum Gene Encoding a 31.3-Kilodalton Endoflagellar Subunit (FlaB2)”,American Society of Microbiology, vol. 57, No. 7,(Jul., 1989),2166-2172.
Pennell, et al., “An Indirect Quantitative Fluorescence Immunoassay for the Detection of Lyme Disease Serum Antibody”,Annals of the NY Academy of Sciences, 539, (1988),483-484.
Plotkin, S. , et al.,In: Vaccines, W.B. Saunders Company, Philadelphia, PA,(1988),p. 571.
Preac-Mursic, V. , et al., “Active Immunization with pC Protein of Borrelia burgdorferi Protects Gerbils against B. burgdorferi Infection”,MMV Medizin Verlag GmbHMunchen, Munchen, (1992),342-349.
Preac-Mursic, V , et al., “European Borrelia burgdorferi Isolated from Humans and Ticks—Culture Conditions and Antibiotic Susceptibility”,Zbl. Bakt. Hyg., A263, (1986),112-118.
Sadziene, Ariadna , et al., “The Cryptic ospC Gene of Borrelia burgdorferi B31 is located on a circular plasmid”,Infection and Immunity, 61, 5, (1993),2192-2195.
Sambrook, J. , et al., “Molecular Cloning”,A Laboratory Manual, Second Addition, Cold Spring Harbor Laboratory Press, 14—In Vitro Amplification of DNA by the Polymerase Chain Reaction,(1989),5 Pages.
Simon, et al., “Recombinant Outer Surface Protein A from Borrelia burgdorferi Induces Antibodies Protective against pirochetal Infection in Mice”,Journal of Infectious Diseases, 164, (Jul. 1991),123-132.
Stern, Peter , “Predicting Antigenic Sites on Proteins”,TIBTECH, 9, (May, 1991),163-167.
Wallich, R , et al., “Clonign and Sequencing of the Gene Encoding the Outer Surface Protein A (OspA) of a European Borrelia burgdorferi isolate”,Nucleic Acids Research, 17, (Nov. 1989),8864.
Wallich, Reinhard , et al., “The Borrelia burgdorferi Flagellum-Associated 41-Kilodalton Antigen (Flagellin): Molecular Cloning, Expression, and Amplification of the Gene”,Infection and Immunity, American Society of for Microbiology,(Jun., 1990),1711-1719.
Wilske, et al., “Antigenic Variability of Borrelia burgdorferi”,Annals of the New York Academy of Sciences, 539, (Aug. 1988),126-143.
Wilske, Bettina , et al., “Detection of IgM-and IgG Antibodies to Borrelia burgdorferi Using Different Strains as Antigen”,Stanek(Ed), Lyme Borrehosis II, Zbl. Bakt Suppl. 18 Gustav Fischer, Stuttgarl, New York, (1989),5 Pages.
Wilske, et al., “Immunochemical Analysis of the Immune Response in Late Manifestations of Lyme Borreliosis”,Zbl. Bakt. Hyg, A 267, (1988),549-558.
Wilske, et al., “Immunochemical and Immunological Analysis of European Borrelia burgdorferi Strains”,International Journal of Microbiology and Hygiene, 263, Series A,(1986),92-102.
Young, et al., “Efficient Isolation of Genes by Using Antibody Probes”,Proceedings of National Academy of Sciences, USA 80, (1983),1194-1198.
Fuchs Renate
Motz Manfred
Preac-Mursic Vera
Soutscheck Erwin
Wilske Bettina
Mikrogen Molekularbiologische Entwicklungs - GmbH
Navarro Mark
Schwegman Lundberg Woessner & Kluth P.A.
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