Immunological reagent and method of using same

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, 1951035A, 210DIG23, 260112R, 260112B, 424 8, 424 11, 424 12, 424 13, 424177, G01N 2100, G01N 2300, G01N 3100, B01D 2101

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041488695

ABSTRACT:
Immunological assaying methods which require insolubilization of an antigen-antibody complex as an integral step thereof are improved by adding to the test medium in which insolubilization is desired an aqueous reagent solution containing from about 3 to 6% by weight of a mixture of polyethylene glycol and a nonionic surfactant, and which solution has a calculated HLB (Hydrophilic-Lipophilic-Balance) of about 0.7 to 1.7. This solution increases the insolubilization of the antigen-antibody complex with reduced incubation times, and produces an assay procedure of greater test range and sensitivity. A preferred embodiment involves usage in a nephelometric analysis.

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