Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-03-14
1993-02-16
Lacey, David L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436821, 436824, 5303871, 53038825, 530412, 530413, 43524027, 435 71, C07K 1500, C07K 318, G01N 3353, C12N 518
Patent
active
051870673
ABSTRACT:
A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, the primary and secondary antibodies having the property of binding to different epitopes of free human protein S, and one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp) but specifically binding to the free human protein S. Also provided is a method of immunologically determining a complex of human protein S and human complement cofactor C4b-binding protein (C4bp) in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to free human protein S and human complement cofactor C4b-binding protein (C4bp) but binding specifically to the complex, and the other being an antibody having the property of binding to the human complement cofactor C4b-binding protein (4Cbp).
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Ichikawa Yataro
Koike Yukiya
Sumi Yoshihiko
Wakabayashi Kenji
Feisee Lila
Lacey David L.
Teijin Limited
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