Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-02-27
1998-03-03
Huff, Sheela
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 72, 435 75, 435 791, 435 792, 435 793, 435 794, 435 795, 435 405, 435174, 435175, 435176, 435177, 435178, 435179, 435180, 435181, 435960, 435972, 436518, 436523, 436524, 436527, 436528, 436529, 436530, 436531, 436532, 436533, 436534, 436536, G01N 3353, G01N 33542, G01N 33537, G01N 33543, G01N 33554, G01N 33551, G01N 33546, G01N 33552
Patent
active
057233047
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
The present invention relates to a method of detection, a sensor and a test-kit which find application in immunological detection (e.g. immunoassay).
SUMMARY OF THE INVENTION
According to one aspect of the present invention there is provided a method of detection, suitable for use in immunological detection of an entity, which method includes the use of a secondary species (as hereinafter defined), the use of a first detectable species, and the use of a second detectable species.
In one embodiment the present invention provides a method of detection, suitable for use in immunological detection of an entity, which method includes the use of a primary species, the use of a secondary species, the use of a first detectable species and the use of a second detectable species.
The primary species may, for example, provide a first detectable species (e.g. when the primary species is a ligand) or the primary species may be capable of being associated with a first detectable species.
The secondary species may, for example, provide a second detectable species (e.g. when the secondary species is a ligand) or the secondary species may be capable of being associated with a second detectable species. (As is further disclosed hereinafter, the secondary species may be, for example, an "auxiliary species", or may be, for example, a species which has a part which may provide an "auxiliary function").
It is to be understood that in this Specification the terms "first" and "second", as applied to detectable species, are used for the purposes of identification and do not imply any order of detection.
The present invention may find application in ratiometric detection (as is further disclosed hereinafter).
A primary species in accordance with the present invention is a species capable of taking part in a primary immune binding reaction. By way of example, a primary immune binding reaction in accordance with the present invention is one in which an entity to be detected undergoes a specific binding reaction or an authentic entity (as hereinafter defined) undergoes a specific binding reaction or an entity to be detected and an authentic entity undergo specific binding reactions. (It will be appreciated that the entity to be detected and the authentic entity undergo specific binding reactions with other species and not with each other.)
By way of example, a primary species may be a primary antibody or a ligand (e.g. an antigen).
It is to be understood that where the primary species is a ligand the primary species may be, for example, an authentic entity.
Also it is to be understood that an "authentic entity" is an entity which is capable of reacting in a substantially similar manner as an entity to be detected under substantially similar conditions. Further, it is to be understood that an authentic entity may, for example, have a chemically defined structure and/or a biologically defined, activity. It will be appreciated that an authentic entity may, for example, be used as a "standard" or a "calibrator". Where, for example, the entity to be detected is itself an analyte species (as hereinafter disclosed) the authentic entity may be an authentic analyte species (or a suitable derivative thereof) which may also be referred to as an "analyte analog".
The secondary species may be, for example, a species which does not itself take part in a primary immune reaction with an entity to be detected (or an authentic entity); such a secondary species may be regarded as an auxiliary species examples of which are auxiliary binders and auxiliary ligands.
Thus, for example, the secondary species may be an auxiliary binder comprising a binder (e.g. an antibody) for an antigenic species or a binder (e.g. a non-antibody) for a non-antigenic ligand such as a binder of a specific ligand-binder pair; an example of a binder for a non-antigenic ligand is avidin.
Examples of secondary species comprising binders for antigenic species are anti-2,4 dinitrophenol antibody, anti-fluorescein antibody, anti-digitoxin antibody, ant
GEC--Marconi Limited
Huff Sheela
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