Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-07-15
2008-07-15
Brusca, John S. (Department: 1631)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C530S388100, C530S388210, C536S024300
Reexamination Certificate
active
10735082
ABSTRACT:
The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably-labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.
REFERENCES:
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4732847 (1988-03-01), Stuart et al.
patent: 4743535 (1988-05-01), Carrico
patent: 4833084 (1989-05-01), Carrico
patent: 4851330 (1989-07-01), Kohne
patent: 4865980 (1989-09-01), Stuart et al.
patent: 5200313 (1993-04-01), Carrico
patent: 5244797 (1993-09-01), Kotewicz et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5455170 (1995-10-01), Abramson et al.
patent: 5510270 (1996-04-01), Fodor et al.
patent: 5668005 (1997-09-01), Kotewicz et al.
patent: 5827661 (1998-10-01), Blais
patent: 5981179 (1999-11-01), Lorinez et al.
patent: 5994079 (1999-11-01), De La Rosa et al.
patent: 6027897 (2000-02-01), Lorincz et al.
patent: 6043038 (2000-03-01), Sivaraja et al.
patent: 6232068 (2001-05-01), Linsley et al.
patent: 6686151 (2004-02-01), De La Rosa et al.
patent: 02 88737 (1988-11-01), None
patent: 0 480 289 (1997-03-01), None
patent: 2 279 954 (1995-01-01), None
patent: 04-4267897 (1992-09-01), None
patent: WO93 10263 (1993-05-01), None
patent: WO 96/24694 (1996-08-01), None
patent: WO 97/10364 (1997-03-01), None
patent: WO 97/31256 (1997-08-01), None
patent: WO97 31256 (1997-08-01), None
patent: WO99 29909 (1999-06-01), None
patent: WO99 32654 (1999-07-01), None
patent: WO99 36571 (1999-07-01), None
patent: WO99 39001 (1999-08-01), None
patent: WO99 40224 (1999-08-01), None
patent: WO 00 60116 (2000-10-01), None
patent: WO 01/36681 (2001-05-01), None
Blais, B.W., 1994 “Transcriptional Enhancement of the Listeria Monocytogenes PCR and Simple Immunoenzymatic Assay of the Product Using Anti-RNA:DNA Antibodies” Appl. Environ. Microbiol. 60:348-352.
Boguslawski et al., 1986 “Characterization of Monoclonal Antibody to DNA:RNA and Its Application to Immunodetection of Hybrids”J. Immunol. Methods89:123-130.
Coutlee et al., 1989 “Comparison of Colorimetric Fluorescent, and Enzymatic Amplification Substrate Systems in an Enzyme Immunoassay for Detection of DNA-RNA Hybrids”J. Clin. Microbiol. 27:1002-1007.
Coutlee et al., 1989 “Immunodetection of DNA with Biotinylated RNA Probes: A Study of Reactivity of a Monoclonal Antibody to DNA-RNA Hybrids” Anal. Biochem. 181:96-105.
Coutlee et al., 1989 “Nonisotopic Detection of RNA in an Enzyme Immunoassay Using a Monoclonal Antibody Against DNA-RNA Hybrids”Anal. Biochem. 181:153-162.
Coutlee et al., 1990 “Quantitative Detection of Messenger RNA by Solution Hybridization and Enzyme Immunoassay” J. Biol. Chem. 265:11601-11604.
Coutlee et al., 1991 “Immunodetection of DNA with Biotinylated RNA Probes: A Study of Reactivity of a Monoclonal Antibody to DNA-RNA Hybrids” Anal. Biochem. 198:217 (Published erratum).
Coutlee et al., 1991 “Detection of Transcripts of Human Papillomaviruses 16 and 18 in Cancer-derived Cell Line and Cervical Biopsies by Enzyme Immunoassay for DNA-RNA Hybrids Following Solution Hybridization” J. Clin. Microbiol. 29:968-974.
Ed M. Southern, “DNA chips: analyzing sequences by hybridization to oligonucleotides on a large scale,” Mar. 1996, TIG, vol. 12, No. 3, pp. 110-115.
Lamourex et al., 1997 “Detection of Campylobacter jejuni in Food and Poultry Viscera Using Immunomagnetic Separation and Microtitre Hybridization,”J. Appl. Microbiol. 83:641-651.
Lazar et al., 1999 “Hybrid Capture® : a Sensitive Signal Amplification-based Chemiluminescent Test for the Detection and Quantitation of Human Viral and Bacterial Pathogens,”J. Clin. Assay22:139-151.
Lennon et al. 1991 Hybridization analyses of arrayed cDNA libraries. Trends in Genetics, vol. 7, pp. 314-317.
Lockhart et al. “Expression monitoring by hybridization to high-density oligonucleotide arrays,” Dec. 1996, Nature Biotechnology, vol. 14, pp. 1675-1680.
Maldonado-Rodriquez et al. “Hybridization of Glass-Tethered Oligonucleotide Probes to Target strands Preannealed with Labeled Auxiliary Oligonucleotides,” 1999, Molecular Biotechnology, vol. 11, pp. 1-12.
Newman et al., 1989 “Solution Hybridization and Enzyme Immunoassay for Biotinylated DNA:RNA Hybrids to Detect Enteroviral RNA in Cell Culture” Mol. Cell Probes 3:375-382.
Stollar, B.D. and A. Rashtchian, 1987 “Immunochemical Approaches to Gene Probe Assays” Anal. Biochem. 161:387-394.
Tominaga et al., “Colorimetric ELISA measurement of specific mRNA on immobilized-oligonucleotide-coated microtiter plates by reverse transcription with biotinylated mononucleotides,” 1996, Clinical Chemistry 42, No. 11, pp. 1750-1757.
Viscidi et al., 1989 “Monoclonal Antibody Solution Hybridization Assay for Detection of Human Immunodeficiency Virus Nucleic Acids” J. Clin. Microbiol. 27:120-125.
International Search Report for corresponding PCT Application No. PCT/US2004/041397.
Broker et al., “A Molecular Portrait of Human Papillomavirus Carcinogenesis”, Cancer Cells, vol. 7, pp. 197-208, 1989.
De Villiers et al., “Classification of Papillomaviruses”, Virology, vol. 324, pp. 17-27, 2004.
Hames & Higgins, Nucleic Acid Hybridisation: A Practical Approach (IRL Press Limited, Oxford, England. 1985).
Heilman et al., “Cloning of Human Papilloma Virus Genomic DNAs and Analysis of Homologous Polynucleotide Sequences”, Journal of Virology, vol. 36, No. 2, pp. 395-407, Nov. 1980.
Hermanson, et al., eds. Immobilized Affinity Ligands (Academic Press, New York), 1992.
Higgins et al., “Transcription Patterns of Human Papillomavirus Type 16 in Genital Intraepithelial Neoplasia: Evidence for Promoter Usage within the E7 Open Reading Frame During Epithelial Differentiation”, Journal of General Virology, vol. 73, pp. 2047-2057, 1992.
Howard et al., “Optimizing the Hybrid Capture II Human Papillomavirus Test to Detect Cervical Intraepithelial Neoplasia”, Obstetrics and Gynecology, vol. 100, No. 5, Part 1, pp. 972-980, Nov. 2002.
Howley et al., “A Rapid Method for Detecting and Mapping Homology between Heterologous DNAs”, Journal of Biological Chemistry, vol. 254, No. 11, pp. 4879-4883, Jun. 10, 1979.
Karlsen et al., “Use of Multiple PCR Primer Sets for Optimal Detection of Human Papillmavirus”, Journal of Clinical Microbiology, pp. 2095-2100, Sep. 1996.
Law et al., “Conserved Polynucleotide Sequences Among the Genomics of Papillomavirus”, Journal of Virology, vol. 32, No. 1, pp. 199-207, Oct. 1979.
Lorincz, A.T., “Molecular Methods for the Detection of Human Papillomavirus Infection”, Obstetrics and Gynecology Clinics of North America, vol. 23, No. 3, pp. 707-730, Sep. 1996.
Mazzuli et al., “Multicenter Comparison of the Digene Hybrid Capture CMV DNA Assay (Version 2.0), the pp65 Antigenemia Assay, and Cell Culture for Detection of Cytomegalovirus Viremia”, J. Clinical Microbiol., vol. 37, No. 4, pp. 958-963, Apr. 1999.
Park et al., “Physical Status and Expression of HPV Genes in Cervical Cancers”, Gynecologic Oncology, vol. 65, pp. 121-129, 1997.
Stoler et al., “Human Papillomavirus Type 16 and 18 Gene Expression in Cervical Neoplasias”, Human Pathology, vol. 23, No. 2, pp. 117-128, Feb. 1992.
Taylor, ed. Protein Immobilization: Fundamentals and Applications
De La Rosa Abel
Lazar James G.
Lorincz Attila T.
Strange Christina M.
Williams Inna R.
Brusca John S.
Digene Corporation
Morgan & Finnegan , LLP
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