Immunological detection of Neisseria bacteria via labelled antib

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, 424 8, 424 12, G01N 3316, A61K 4300

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active

041883713

ABSTRACT:
This invention is concerned with two closely related assay methods for detecting the presence of Neisseria bacteria in a fluid sample. Both of the methods utilize radiolabelled antibodies specific to the enzyme released upon lysis of the bacteria. In the first method, denominated immunoradiometric assay (IRMA), the enzyme is reacted with soluble purified radioactive antibodies. In the second method, known variously as "two-site IRMA", "junction test", or "sandwich technique", contemplates initial insolubilization of the enzyme and thereafter the reaction with soluble purified radioactive antibodies.
The structure and composition of the enzyme released upon lysis of Neisseria bacteria are not fully comprehended but it has the capability of oxidizing 1,2-propanediol and reducing nicotinamide-adenine-dinucleotide (NAD). This has led to the name 1,2-propanediol dehydrogenase being proposed for the enzyme.

REFERENCES:
patent: 3974269 (1976-08-01), Maley
patent: 4029756 (1977-06-01), Gaafar
patent: 4066744 (1978-01-01), Price et al.
Tramont et al., Journal of Infectious Diseases, vol. 130, No. 3, Sep. 1974, pp. 240-247.

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