Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-04-29
1996-11-12
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436815, G01N 33545
Patent
active
055739229
ABSTRACT:
An immunological method for the determination of triazine and triazine derivatives by a competitive immunoassay in which the sample and labelled atrazine compete for a polyclonal antibody which is bound to a solid phase before, during or after the immunological reaction, the immunological complex binds to the solid phase, the solid phase and unbound labelled atrazine are separated and the label in the solid or liquid phase is determined as a measure of the content of triazine and triazine derivatives, which is characterized in that polyclonal antibodies which were obtained by immunization with a 4-alkylamino-S-triazine are used and a conjugate of a label and 4-amino-S-triazine which is bound to the label via the 6 position is used as the labelled atrazine. Triazine and triazine derivatives can be determined simultaneously using this method.
REFERENCES:
patent: 4530786 (1985-07-01), Dunbar et al.
Vom Wasser, Bd. 75, pp. 115-126, "ELISAs for the Analysis of Atrazine and Atrazine Metabolites in Water", 1990.
Harrison et al., Journal of Agricultural and Food Chemistry, Bd. 39, Nr. 1, 1991, "Competitive Inhibition ELISA for the S-triazine Herbicides: Assay Optimization and Antibody Characterization", Wash, DC., pp. 122-128.
Wittmann et al., Journal of Agricultural and Food Chemistry, Bd. 39, Nr. 6, 1991, "Development of an ELISA for the Analysis of Atrazine Metabolites Deethylatrazine and Deisopropylatrazine", Wash., DC., pp. 1194-1200.
Dunbar et al., Journal of Agricultural and Food Chemistry, Bd. 39, Nr. 2, 1990, pp. 433-437, "Development of Enzyme Immunoassay for the Detection of Triazine Herbicides", Wash., DC.
Huber et al., Zeitschrift Fur Pflanzenkrankheiten Und Pflanzenschutz, Bd. 92, Nr. 2, 1985, pp. 147-156, "A Solid-phase Enzyme Immunoassay for Quantitative Determination of the Herbicide Terbutryn", Stuttgart, DE.
P. Kramer et al., Pestic. Sci., vol. 32, No. 4, pp. 451-462 (1991).
C. Wittmann et al., Vom Wasser, vol. 75, pp. 115-126 (1990).
C. Wittmann et al., Food Agric. Immunol., vol. 1, No. 4, pp. 211-224 (1989).
A. Lucas et al., Food Agric. Immunol., vol. 3, No. 3-4, pp. 155-167 (1991).
A. Lucas et al., Chem. Res. Toxicol., vol. 6, No. 1, pp. 107-116 (1993).
U. Obst, "Application of Molecular Biological Methods in Water Analysis using Spectrometric Detection", GIT Fachz. Lab. 4 (1992) 365.
R. Reupert, "Analytical Method for the Determination of Pesticides by Micro-HPLC", GIT Spezialchromatographie 1 (1992) 30-36.
L. Weil, "Die Problematik der Spurenanalytik, von Pflanzenschutzmitteln im Trinkwasser und in Gewassern", Nachr. Chem. Techn. Lab. 39 (1991) 1277.
B. Dunbar et al., "Development of Enzyme Immunoassay for the Detection of Triazine Herbicides", J. Agric. Food Chem. 38 (1990) 433-437.
M. H. Goodrow et al., "Hapten Synthesis, Antibody Development, and Competitive Inhibition Enzyme Immunoassay for s-Triazine Herbicides", J. Agric. Food Chem. 38 (1990) 990-996.
S. Wust et al., "Sensitive s-Triazine Enzyme Immunoassay for Water Samples in Polysterene Tubes", GIT Fachz. Labor 2 (1990) 99.
S. J. Huber et al., "Solid-Phase Enzyme Immunoassay for the Determination of Herbicides from Fresh Water--Polystyrene Spheres in Comparison to Microtiter Plates as Antibody Carriers", GIT Fachz. Labor, 10 (1985) 969.
Hoess Eva
Huber Erasmus
Markert-Hahn Christine
Rollinger Wolfgang
Boehringer Mannheim GmbH
Ceperley Mary E.
LandOfFree
Immunological detection method for triazines does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunological detection method for triazines, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunological detection method for triazines will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-561725