Immunoglobulin adsorption

Details Immunoglobulin adsorption Immunoglobulin adsorption

1986-10-31

1989-11-14

Rosen, Sam

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436512, 436519, 436525, 436527, 436529, 436530, 436531, 436823, 435 7, 530388, G01N 3353, G01N 33543, G01N 33563, G01N 33544

Patent

active

048807516

ABSTRACT:
The present disclosure is directed to methods for the preparation of immunoadsorption matrices having IgG molecules adsorbed thereto in a preferred configuration, i.e., adsorbed to the matrix by their (Fc) rather than F(ab) portions. IgG molecules, are selected such that the F(ab) portion of the IgG fraction adsorbed has a more acidic or basic net isoelectric point or pI range than the F(c) end of the molecule, depending on the characteristics of the adsorption surface. For negatively charged surfaces, IgG molecules having relatively alkaline F(c) portions are selected. For positively charged surfaces, IgG with relatively acidic F(c) portions are selected. Additional selection criteria include pI fractionation to provide fractions having well defined pI characteristics as defined by "non-overlap" or "pI range" of F(c) and F(ab) portions pI's. Methods disclosed are particularly well suited to the preparation of colloidal gold immunostains.

REFERENCES:
patent: 4145406 (1979-03-01), Schick et al.
patent: 4208479 (1980-06-01), Zuk et al.
patent: 4313734 (1982-02-01), Leuvering
patent: 4446238 (1984-05-01), DeMey et al.
Faulk et al. (1971), Immunochemistry, 8:1081.
Romano et al. (1974), Immunochemistry, 11:521.
Horisberger et al. (1975), Experientia, 31:1147.
Bauer et al. (1975), Experientia, 31:1149.
Horisberger et al. (1977), Jrnl. Histochem. Cytochem., 25:295.
Geoghegan et al. (1978), Immunol. Comm., 7:1.
Geoghegan et al. (1980), Jrnl. Immunol. Meth. 34:11.
Geoghegan et al. (1981), Immunology, 44:331.
Goodman et al. (1981), Jrnl. Microscopy, 123:201.
DeMey et al. (1981), Prot. Biol. Fluids, pp. 173-176.
DeMey et al. (1981), Cell Biol. Intl. Rep., 5:889.
Warchol et al. (1982), Histochemistry, 76:567.
DeWaele et al. (1983) in Techniques in Immunocytochemistry, Vol. 2, pp. 1-22.
DeMey (1983) in Immunochemistry: Practical Applications in Pathology and Biology, pp. 82-112, Eds. J. Polak and S. Van Noorden.
DeMey (1983), in Immunochemistry, pp. 347-369, Ed. A. C. Cuello.
Handley et al. (1983), Proceedings Society Exp. Biol. and Med., 174:1-2.
Tinglu et al. (1984) Jrnl. Bacteriol., 159:668.
Slot et al. (1984) in Immunolabelling for Electron Microscopy, pp. 129-141.
Colloidal Metal Marking Reference Book (1984), pp. 1-7.
Wang et al. (1985), Histochemistry, 83:109.
Geoghegan (1985), Jrn. Cell Biol. 101:85A, presented at 25th Annual ASCB Meeting, Nov. 18-22, 1985.
Poster presented at 25th Annual ASCB Meeting, Nov. 18-22, 1985.
Park et al. (1986), Jrnl. Colloid Interface Sci., 111:197.
Dialog Search Report.
Geoghegan et al. (1977), Jrnl. Hist. Cytochem., 25:1187.
Bagchi and Birnbaum, J. Colloid and Interface Science, 83:460-478 (1981).
Morrissey and Hand, J. Colloid and Interface Science, 65:423-431 (1978).
Handley and Chien, Proceedings of the Society for Experimental Biology and Medicine, 174:1-11 (1983).

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