Immunogenic ryanodine derivative and related uses

Details Immunogenic ryanodine derivative and related uses Immunogenic ryanodine derivative and related uses

1994-01-25

1996-02-20

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436545, 436822, 530363, 530367, 530380, 5303898, 530404, 530405, G01N 33567, G01N 33534, C07K 1476

Patent

active

054928397

ABSTRACT:
Disclosed is a novel photo-activatable derivative of ryanodine 9-hydroxy-21-(4-azidobenzoyloxy)-9-epiryanodine. This novel ryanodine derivative has been conjugated to keyhole limpet hemocyanin (KLH) and used for the production of antibodies with high affinity and specificity to ryanodine. A radioimmunoassay specific for ryanodine was developed using the anti-ryanodine antibodies, and a dissociation constant for ryanodine of 1 nM was determined. Also disclosed is the binding of a labeled, photoactivatable derivative of ryanodine (ABRy) with the ryanodine receptor of skeletal, cardiac and brain membranes. Digestion of the labeled ryanodine receptor revealed a labeled 76 kDa tryptic fragment which is critical for the formation of the high affinity ryanodine binding site.

REFERENCES:
G. Liu et al., Mol. Pharmacology, vol. 45, No. 2, pp. 189-200 (1994).
J. Gosling, Clin. Chem., vol. 36, No. 8, pp. 1408-1427 (1990).
G. Liu et al., Chem. Abstract No. 120:240990n (1994).
A. Caswell et al., Biochemistry, vol. 30, No. 30, pp. 7507-7513 (1991).
S. Kahl et al., Anal. Biochem., vol. 218, No. 1, pp. 55-62 (1994).

Affiliated with

Also associated with

Rating

Immunogenic ryanodine derivative and related uses does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunogenic ryanodine derivative and related uses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunogenic ryanodine derivative and related uses will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1355635