Immunogenic protein or peptide complex, method of producing said

Drug – bio-affecting and body treating compositions – Lymphokine

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530300, 530344, 530350, 530403, 530406, 530419, 530423, 424 89, 424 92, 435 68, 4351722, 4351723, A61K 3900, C07K 102, C07K 312, C07K 1710

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047449837

ABSTRACT:
The invention relates to an immunogenic complex containing antigenic membrane proteins or peptides from viruses mycoplasmas, bacteria, parasites or animal cells or prepared synthetically or with hybride DNA technique and glycoside. The invention also relates to a process for producing the complex, whereby microorganisms, animal cells, proteins and peptides are mixed with solubilizing agents in buffered, possibly saline, solution, whereby complexes are formed between charged monomeric antigenic proteins and detergent or peptides and solubilizing agents, whereafter the charged monomeric antigenic proteins or peptides are separated from solubilizing agents in the presence of, or are separated from solubilizing agent and directly transferred to, a glycoside solution which contains one or more glycosides with hydrophobic and hydrophilic regions in a concentration of at least the critical micellular concentration, whereby a protein complex is formed, which is isolated and purified. The invention also relates to the use of the immunogenic complex as an immunity-stimulating agent, especially as a vaccine, and compositions containing the complex.

REFERENCES:
patent: 4053585 (1977-10-01), Allison
patent: 4064232 (1977-12-01), Bachmayer
patent: 4117113 (1978-09-01), Allison
patent: 4148876 (1979-04-01), Almeida
patent: 4196191 (1980-04-01), Almeida
patent: 4201767 (1980-05-01), Fullerton
patent: 4235877 (1980-11-01), Fullerton
patent: 4251509 (1981-02-01), Hanson
patent: 4261975 (1981-04-01), Fullerton
patent: 4356169 (1982-10-01), Simons
patent: 4578269 (1986-03-01), Morein
Chemical Abstracts, 176074p, vol. 81, p. 428, 1974.
Arch. Gasamte Virusforsch, 1974, 44 (3), 243-254, Dalsgaard.

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