Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...
Patent
1984-08-01
1987-11-17
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Plant proteins, e.g., derived from legumes, algae or...
530395, 530417, 530415, 530806, 530826, 424 89, 436820, 210635, 2105021, 2101982, 435 5, 435 68, 435 70, 4351723, 435235, 435239, 435255, 435803, A61K 3929, C08B 3712, C12N 1500, C07K 320
Patent
active
047075422
ABSTRACT:
The surface antigen protein of human Hepatitis B virus is synthesized in Saccharomyces cerevisiae as a 23,000-26,000 dalton polypeptide, essentially free of intermolecular disulfide bonds. This antigen is a poor immunogen in animals and man. No prior precedent or method exists for efficiently converting the non-disulfide bonded antigen to a fully intermolecular disulfide bonded particle. We describe the first example of such a conversion in vitro and show that the act of this conversion enhances the immunogenicity of the antigen about 10-fold. The in vitro conversion makes practical the production of hepatitis B surface antigen from microorganisms using recombinant DNA methods.
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Friedman Arthur
Lehman E. Dale
McAleer William J.
Schaefer Ted F.
Scolnick Edward M.
Merck & Co. , Inc.
Moskowitz Margaret
Perrella Donald J.
Pfeiffer Hesna J.
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