Immunogenic detoxified mutants of cholera toxin and of the toxin

Details Immunogenic detoxified mutants of cholera toxin and of the toxin Immunogenic detoxified mutants of cholera toxin and of the toxin

1997-03-25

2000-11-21

Housel, James C.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

4241841, 4242341, 4242401, 4242411, 4242571, 4242611, 435 693, 4351723, A61K 3902, A61K 39108, A61K 39106, C12P 2106

Patent

active

061499196

ABSTRACT:
An immunogenic detoxified protein comprising the amino acid sequence of subunit A of cholera toxin (CT-A) or subunit A of an Escherichia coli heat labile toxin (LT-A) or a fragment thereof wherein one or more amino acids at, or in positions corresponding to Val-53, Ser-63, Val-97, Tyr-104 or Pro-106 are replaced with another amino acid or deleted. Examples of specific replacements include Val-53-Asp, Val-53-Glu, Val-53-Tyr, Ser-63-Lys, Val-97-Lys, Val-97-Tyr, Tyr-104-Lys, Tyr-104-Asp, Tyr-104-Ser, Pro-106-Ser. The immunogenic detoxified protein is useful as vaccine for Vibrio cholerae or an enterotoxigenic strain of Escherichia coli and is produced by recombinant DNA means by site-directed mutagenesis.

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