Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Reexamination Certificate
2006-09-12
2006-09-12
Minnifield, Nita (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
C435S071200, C435S071300, C435S261000, C435S308100, C424S253100, C424S254100, C424S282100, C424S093300, C424S093400
Reexamination Certificate
active
07105317
ABSTRACT:
This invention relates to an immunogenic composition comprising a bacterial extract containing at least one expression products of the vrg genes of a strain ofBordetellachosen fromB. pertussis, B. parapertussis, orB. bronchiseptica. The invention also relates to a method for producing the extract, comprising culturing the bacteria on blood medium to obtain isolated nonhemolytic colonies; inoculating cells of one or more colonies in liquid medium to give a suspension of cells; separating the cells from the liquid medium after culture; suspending the separated cells in a buffer comprising urea for at least an amount of time sufficient to form a bacterial lysate; and separating intact cells and insoluble material from soluble material, wherein the extract comprises the soluble material.
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Gueirard Pascale
Guiso Nicole
Finnegan Henderson Farabow Garrett & Dunner LLP
Institut Pasteur
Minnifield Nita
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