Immunogenic cell envelope preparations

Details Immunogenic cell envelope preparations Immunogenic cell envelope preparations

1979-02-16

1981-02-03

Rose, Shep K.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

A61K 3910

Patent

active

042488621

ABSTRACT:
An antigenically-active cell envelope fraction from bacterial cells, especially Bordetella pertussis, may be isolated by disrupting the cells, separating the cell envelopes from the other cell components, extracting the separated envelopes with a solution of a zwitterionic surfactant and precipitating the cell envelope preparation from the resulting extract by the addition of a lower alkyl (C.sub.1 to C.sub.5) alcohol, preferably ethanol. The preferred extractant is an N, N, N-trialkyl zwitterionic compound especially an N, N, N-trialkyl-.alpha.-amino acid or an N, N, N-trialkyl amine oxide.
The cell envelope fraction obtained from B. pertussis is highly protective against whole bacteria when tested by standard vaccine mouse potency assay and has a greatly reduced lipopolysaccharide content compared with either whole cells or crude cell envelopes, greatly reducing the risk of undesirable side-effects from vaccination.

REFERENCES:
patent: 2965543 (1960-12-01), Thiele
patent: 3141824 (1964-07-01), Dahlstrom
patent: 3395219 (1968-07-01), Millman
Robinson, A. et al., FEMS Microbiol. Lett (1979) 5(3): 131-134, Solubilization of the Protective Antigens of Bordetella pertussis`, CA. 90#174558M (1979).
Dyke et al., J. Med. Virol., 1978, 2(2): 143-152 Glycoproteins of Representative Paramyxoviruses Isolation and Antigenic Analysis Using a Zwitterionic Surfactant, CA. 89#102953T (1978).
Gow et al. Microbios. (1976):15(61-62):209-219, Radiolabeling of Bordetella pertussis Envelope Proteins by .sup.125 I-Lactoperoxidase, CA. 86#52631b (1977).
Parton et al., CA. 834600g, 1975 of J. Med Microbiol, 1975, 8(1):47-57, Cell Envelope Proteins of Bordetella pertussis.
Wardlaw et al., CA. 85 2260p (1976) of J. Med. Microbiol. 1976 9(1):89-100 Loss of . . . Envelope Polypeptides in Cultural Variants of Bordetella pertussis.
Allen et al., FEBS Letters 57(2):158-162, Sep. 1975, The Use of Zwitterionic Surfactants in the Agarose Chromatography of Biological Membranes.

Affiliated with

Also associated with

Rating

Immunogenic cell envelope preparations does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunogenic cell envelope preparations, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunogenic cell envelope preparations will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-550900