Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-05-13
1994-12-20
Bidwell, Carol E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 435962, 435967, G01N 33569
Patent
active
053745302
ABSTRACT:
An ELISA method with competitive inhibition is described for determining antisporozoite antibodies of P. Falciparum in human blood samples and in mosquito extract, which uses a single plate pretreated with only the synthetic antigen (NANP).sub.20 using, as total competitive inhibitor for the formation of the complex between the synthetic antigen adsorbed on the plate and the antibody contained in the sample, the (NANP).sub.20 synthetic peptide in a weight ratio of at least 20:1 with respect to the adsorbed antigen. Because of its high specificity, sensitivity and speed, the method is particularly suitable for epidemiological studies on malaria.
REFERENCES:
Giuseppe Del Giudice et al., Detection of Human Antibodies Against Plasmodium Falciparum Sporozoites Using Synthetic Peptides, Journal of Clinical Microbiology, Jan., 1987, pp. 91-96.
Enzyme-Linked Immunosorbent Assay (ELISA) Theoretical and Practical Aspects, Clark et al., pp. 167-180.
Vogt et al., Quantitative Differences Among Various Proteins as Blocking Agents For ELISA Microtiter Plates, Journal of Immunological Methods, vol. 101, 1987, pp. 43-50.
C. Burrells et al., Part 1: Fundamental Aspects of ELISA Technology, ELISA METHODOLOGY: Variations in Technical Procedures, pp. 9-2.
Immunohistochemistry, A. C. Cuello, editor, pp. 8-4, John Wiley & Sons (Chichester), 1983.
Antibodies, A Laboratory Manual, Harlow et al, pp. 313-315 "Immunoaffinity Purification of Antibodies on an Antigen Column", Cold Springs Harbor Laboratory, 1988.
Bernardi Adriano
Nuzzolo Carlo A.
Pessi Antonello
Verdini Antonio S.
Bidwell Carol E.
Eniricerche S.p.A.
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